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| Main Authors: | , |
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| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2021
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.930105 |
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| _version_ | 1867171874598813696 |
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| author | Adam, Nicole Perner, Mirjam |
| author_facet | Adam, Nicole Perner, Mirjam |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_930105 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2 Adam, Nicole Perner, Mirjam Accession number, genetics; Area/locality; Event label; function-based screen; Hydrogenase; Hydrogen uptake activity; Hydrogen uptake activity, standard deviation; hydrothermal vent; M78/2; M78/2_274; M78/2_314; M78/2_324; MARSUED5; Metagenome; Meteor (1986); Remote operated vehicle; ROV; Sample ID; Sample method Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. |
| title | Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2 |
| topic | Accession number, genetics; Area/locality; Event label; function-based screen; Hydrogenase; Hydrogen uptake activity; Hydrogen uptake activity, standard deviation; hydrothermal vent; M78/2; M78/2_274; M78/2_314; M78/2_324; MARSUED5; Metagenome; Meteor (1986); Remote operated vehicle; ROV; Sample ID; Sample method |
| url | https://doi.org/10.1594/PANGAEA.930105 |