TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2021
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| _version_ | 1867171875108421632 |
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| author | Hornick, Thomas Mach, Elke Grossart, Hans-Peter |
| author_facet | Hornick, Thomas Mach, Elke Grossart, Hans-Peter |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We simulated an experimental summer storm in large-volume (~1200 m³, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_930921 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria Hornick, Thomas Mach, Elke Grossart, Hans-Peter 14C-leucine incorporation; Bacteria, free-living; Bacterial abundance of HDNA-Bacteria; Bacterial abundance of LDNA-Bacteria; bacterial production; BIBS; Bridging in Biodiversity Science; chlorophyll; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; Day of the year; DCM; DEPTH, water; derived; Description; disturbance; enclosure experiment; Enclosure experiment; Epifluorescence microscopy after DAPI staining; Flow cytometry; Germany; Lake; Lake_Stechlin; mesocosm experiment; Mesocosm label; NITROLIMIT; Nostocales biovolume; Nostocales cell abundance; Particle-associated bacterial abundance; Picocyanobacteria; Protein production, free-living bacteria; Protein production, particle associated bacteria; Stickstofflimitation in Binnengewässern; summer storm; TemBi; Treatment We simulated an experimental summer storm in large-volume (~1200 m³, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures. |
| title | TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria |
| topic | 14C-leucine incorporation; Bacteria, free-living; Bacterial abundance of HDNA-Bacteria; Bacterial abundance of LDNA-Bacteria; bacterial production; BIBS; Bridging in Biodiversity Science; chlorophyll; Climate change; Climate driven Changes in Biodiversity of Microbiota; cyanobacteria; DATE/TIME; Day of experiment; Day of the year; DCM; DEPTH, water; derived; Description; disturbance; enclosure experiment; Enclosure experiment; Epifluorescence microscopy after DAPI staining; Flow cytometry; Germany; Lake; Lake_Stechlin; mesocosm experiment; Mesocosm label; NITROLIMIT; Nostocales biovolume; Nostocales cell abundance; Particle-associated bacterial abundance; Picocyanobacteria; Protein production, free-living bacteria; Protein production, particle associated bacteria; Stickstofflimitation in Binnengewässern; summer storm; TemBi; Treatment |
| url | https://doi.org/10.1594/PANGAEA.930921 |