Activities of key metabolic enzymes and their respective mRNA transcript levels in amphipods exposed to an experimental warming scenario
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2021
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| author | Jakob, Lena Vereshchagina, Kseniya P Tillmann, Anette Rivarola-Duarte, Lorena Axenov-Gribanov, Denis V Bedulina, Daria S Gurkov, Anton N Drozdova, Polina Timofeyev, Maxim A Stadler, Peter F Luckenbach, Till Pörtner, Hans-Otto Sartoris, Franz-Josef Lucassen, Magnus |
| author_facet | Jakob, Lena Vereshchagina, Kseniya P Tillmann, Anette Rivarola-Duarte, Lorena Axenov-Gribanov, Denis V Bedulina, Daria S Gurkov, Anton N Drozdova, Polina Timofeyev, Maxim A Stadler, Peter F Luckenbach, Till Pörtner, Hans-Otto Sartoris, Franz-Josef Lucassen, Magnus |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Lake Baikal is inhabited by more than 300 endemic amphipod species, which are narrowly adapted to certain thermal niches due to the high interspecific competition. In contrast, the surrounding freshwater fauna is commonly represented by species with large-scale distribution and high phenotypic thermal plasticity. Here, we investigated the thermal plasticity of the energy metabolism in two closely-related endemic amphipod species from Lake Baikal (Eulimnogammarus verrucosus; stenothermal and Eulimnogammarus cyaneus; eurythermal) and the ubiquitous Holarctic amphipod Gammarus lacustris (eurythermal) by exposure to a summer warming scenario (6-23.6 °C; 0.8 °C d-1). In concert with routine metabolic rates, activities of key metabolic enzymes increased strongly with temperature up to 15 °C in E. verrucosus, whereupon they leveled off (except for lactate dehydrogenase). In contrast, exponential increases were seen in E. cyaneus and G. lacustris throughout the thermal trial (Q10-values: 1.6-3.7). Cytochrome-c-oxidase, lactate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase activities were found to be higher in G. lacustris than in E. cyaneus, especially at the highest experimental temperature (23.6 °C). Decreasing gene expression levels revealed some thermal compensation in E. cyaneus but not in G. lacustris. In all species, shifts in enzyme activities favored glycolytic energy generation in the warmth. The congruent temperature-dependencies of enzyme activities and routine metabolism in E. verrucosus indicate a strong feedback-regulation of enzymatic activities by whole organism responses. The species-specific thermal reaction norms reflect the different ecological niches, including the spatial distribution, distinct thermal behavior such as temperature-dependent migration, movement activity, and mating season. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_931524 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Activities of key metabolic enzymes and their respective mRNA transcript levels in amphipods exposed to an experimental warming scenario Jakob, Lena Vereshchagina, Kseniya P Tillmann, Anette Rivarola-Duarte, Lorena Axenov-Gribanov, Denis V Bedulina, Daria S Gurkov, Anton N Drozdova, Polina Timofeyev, Maxim A Stadler, Peter F Luckenbach, Till Pörtner, Hans-Otto Sartoris, Franz-Josef Lucassen, Magnus 3-hydroxyacyl-CoA dehydrogenase (HADH); Amphipoda; Bolshie_Koty; citrate synthase (CS); cytochrome-c-oxidase (COX); glutamate dehydrogenase (GDH); HAND; lactate dehydrogenase (LDH); Lake-14; Lake Baikal; metabolic fuel use; pyruvate kinase (PK); Sampling by hand; Temperature; thermal adaption Lake Baikal is inhabited by more than 300 endemic amphipod species, which are narrowly adapted to certain thermal niches due to the high interspecific competition. In contrast, the surrounding freshwater fauna is commonly represented by species with large-scale distribution and high phenotypic thermal plasticity. Here, we investigated the thermal plasticity of the energy metabolism in two closely-related endemic amphipod species from Lake Baikal (Eulimnogammarus verrucosus; stenothermal and Eulimnogammarus cyaneus; eurythermal) and the ubiquitous Holarctic amphipod Gammarus lacustris (eurythermal) by exposure to a summer warming scenario (6-23.6 °C; 0.8 °C d-1). In concert with routine metabolic rates, activities of key metabolic enzymes increased strongly with temperature up to 15 °C in E. verrucosus, whereupon they leveled off (except for lactate dehydrogenase). In contrast, exponential increases were seen in E. cyaneus and G. lacustris throughout the thermal trial (Q10-values: 1.6-3.7). Cytochrome-c-oxidase, lactate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase activities were found to be higher in G. lacustris than in E. cyaneus, especially at the highest experimental temperature (23.6 °C). Decreasing gene expression levels revealed some thermal compensation in E. cyaneus but not in G. lacustris. In all species, shifts in enzyme activities favored glycolytic energy generation in the warmth. The congruent temperature-dependencies of enzyme activities and routine metabolism in E. verrucosus indicate a strong feedback-regulation of enzymatic activities by whole organism responses. The species-specific thermal reaction norms reflect the different ecological niches, including the spatial distribution, distinct thermal behavior such as temperature-dependent migration, movement activity, and mating season. |
| title | Activities of key metabolic enzymes and their respective mRNA transcript levels in amphipods exposed to an experimental warming scenario |
| topic | 3-hydroxyacyl-CoA dehydrogenase (HADH); Amphipoda; Bolshie_Koty; citrate synthase (CS); cytochrome-c-oxidase (COX); glutamate dehydrogenase (GDH); HAND; lactate dehydrogenase (LDH); Lake-14; Lake Baikal; metabolic fuel use; pyruvate kinase (PK); Sampling by hand; Temperature; thermal adaption |
| url | https://doi.org/10.1594/PANGAEA.931524 |