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| Main Authors: | , , , |
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| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2021
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.931663 |
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| _version_ | 1867172342094888960 |
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| author | Müller, Carolina Hennig, Julian Riedel, Frank Helle, Gerhard |
| author_facet | Müller, Carolina Hennig, Julian Riedel, Frank Helle, Gerhard |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Purification protocols to extract pollen from lake sediments contain chemicals, which alter the carbon and oxygen pollen-isotope values according to pollen characteristics and family affiliation. Modern (raw) pollen of broad-leaved (Alnus glutinosa, Betula pendula, Carpinus betulus, Corylus avellana, Fagus sylvatica and Quercus robur) and coniferous tree species (Picea abies and Pinus sylvestris) were treated with potassium hydroxide (KOH), hydrofluoric acid (HF), sodium hypochlorite (NaClO) and sulphuric acid (H2SO4) to test the impact on δ13Cpollen and δ18Opollen and assess the applicability in purification protocols. Alterations of δ13Cpollen values vary between +1.0‰ (B. pendula, NaClO-treatment) and -5.0‰ (P. sylvestris, H2SO4-treatment). The impact of chemicals on δ18Opollen are more diverse and offsets range between +1.1‰ (C. avellana, NaClO-treatment) and -17.9‰ (P. sylvestris, H2SO4-treatment). Pollen of broad-leaved and coniferous trees reacted differently to chemicals, but response patterns are generally alike. The usage of isotope-altering chemicals in purification protocols should be brought to a minimum, but the application of KOH and NaClO seems unproblematic. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_931663 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Impact of chemicals on stable carbon and oxygen isotope values of raw pollen Müller, Carolina Hennig, Julian Riedel, Frank Helle, Gerhard Biological sample; BIOS; chemical treatment; ELEVATION; LATITUDE; LONGITUDE; Pollen; pollen_experiment_exctraction; pollen purification protocol; Replicates; Sample ID; Species; Stable carbon and oxygen isotopes; Treatment; δ13C, pollen; δ18O, pollen Purification protocols to extract pollen from lake sediments contain chemicals, which alter the carbon and oxygen pollen-isotope values according to pollen characteristics and family affiliation. Modern (raw) pollen of broad-leaved (Alnus glutinosa, Betula pendula, Carpinus betulus, Corylus avellana, Fagus sylvatica and Quercus robur) and coniferous tree species (Picea abies and Pinus sylvestris) were treated with potassium hydroxide (KOH), hydrofluoric acid (HF), sodium hypochlorite (NaClO) and sulphuric acid (H2SO4) to test the impact on δ13Cpollen and δ18Opollen and assess the applicability in purification protocols. Alterations of δ13Cpollen values vary between +1.0‰ (B. pendula, NaClO-treatment) and -5.0‰ (P. sylvestris, H2SO4-treatment). The impact of chemicals on δ18Opollen are more diverse and offsets range between +1.1‰ (C. avellana, NaClO-treatment) and -17.9‰ (P. sylvestris, H2SO4-treatment). Pollen of broad-leaved and coniferous trees reacted differently to chemicals, but response patterns are generally alike. The usage of isotope-altering chemicals in purification protocols should be brought to a minimum, but the application of KOH and NaClO seems unproblematic. |
| title | Impact of chemicals on stable carbon and oxygen isotope values of raw pollen |
| topic | Biological sample; BIOS; chemical treatment; ELEVATION; LATITUDE; LONGITUDE; Pollen; pollen_experiment_exctraction; pollen purification protocol; Replicates; Sample ID; Species; Stable carbon and oxygen isotopes; Treatment; δ13C, pollen; δ18O, pollen |
| url | https://doi.org/10.1594/PANGAEA.931663 |