The abundance of soil fungi with associated functional and taxonomic guilds in pure and mixed beech-conifer tree stands
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| Autores principales: | , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2021
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| _version_ | 1867171027655589888 |
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| author | Likulunga, Likulunga Emmanuel Rivera Pérez, Carmen Alicia Schneider, Dominik Daniel, Rolf Polle, Andrea |
| author_facet | Likulunga, Likulunga Emmanuel Rivera Pérez, Carmen Alicia Schneider, Dominik Daniel, Rolf Polle, Andrea |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This dataset provides operational taxonomic units (OTU) abundance table of soil fungi with assigned taxonomic and functional guilds. The soil samples were collected in pure and beech-conifer mixed tree stands in Germany in November/December 2017, at 10 cm depth after removal of non-decomposed litter layer. The tree stands and site descriptions are provided (Ammer et al., 2020; Glatthorn, 2020). DNA was extracted from the samples using the DNeasy® PowerSoil® Pro kit (Qiagen, Hilden, Germany) and purified with DNeasy® PowerClean® kit (Qiagen, Hilden, Germany). Amplification (PCR) was achieved by using primers Mi_ITS3KYO2 (1:10) (Toju et al., 2012) and Mi_ITS4 (1:10) (White et al., 1990). The purification, pooling and quantification of PRC reactions were done as described (Clausing et al., 2020). Illumina sequencing was conducted by the Göttingen Genomics Laboratory (G2L) on MiSeq Platform using MiSeq Reagent Kit v3 (Illumina Inc., San Diego, USA). Raw paired - end reads (fasta.gz files: forward and reverse) were analyzed with MiSeq Marker gene pipeline V1.9. The abundance table contains 97% similarity fungal sequences rarefied to same number of counts (13668). Functional annotation of fungi was done using the FUNGuild database (Nguyen et al., 2016). The raw data were deposited in NCBI (National Center for Biotechnology Information) SRA (Sequence Read Archive) with bioproject accession number: PRJNA704813. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_933499 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | The abundance of soil fungi with associated functional and taxonomic guilds in pure and mixed beech-conifer tree stands Likulunga, Likulunga Emmanuel Rivera Pérez, Carmen Alicia Schneider, Dominik Daniel, Rolf Polle, Andrea beech-conifer stands; Binary Object; Binary Object (File Size); Binary Object (Media Type); Dassel; Enrichment of European beech forests with conifers; functional guilds; Germany; Goehrde_I; Goehrde_II; Harz; Nienburg; Nienover; OTU abundance; RTG2300; RTG2300-I; soil fungi; Unterluess; VID; Visual identification; Winnefeld This dataset provides operational taxonomic units (OTU) abundance table of soil fungi with assigned taxonomic and functional guilds. The soil samples were collected in pure and beech-conifer mixed tree stands in Germany in November/December 2017, at 10 cm depth after removal of non-decomposed litter layer. The tree stands and site descriptions are provided (Ammer et al., 2020; Glatthorn, 2020). DNA was extracted from the samples using the DNeasy® PowerSoil® Pro kit (Qiagen, Hilden, Germany) and purified with DNeasy® PowerClean® kit (Qiagen, Hilden, Germany). Amplification (PCR) was achieved by using primers Mi_ITS3KYO2 (1:10) (Toju et al., 2012) and Mi_ITS4 (1:10) (White et al., 1990). The purification, pooling and quantification of PRC reactions were done as described (Clausing et al., 2020). Illumina sequencing was conducted by the Göttingen Genomics Laboratory (G2L) on MiSeq Platform using MiSeq Reagent Kit v3 (Illumina Inc., San Diego, USA). Raw paired - end reads (fasta.gz files: forward and reverse) were analyzed with MiSeq Marker gene pipeline V1.9. The abundance table contains 97% similarity fungal sequences rarefied to same number of counts (13668). Functional annotation of fungi was done using the FUNGuild database (Nguyen et al., 2016). The raw data were deposited in NCBI (National Center for Biotechnology Information) SRA (Sequence Read Archive) with bioproject accession number: PRJNA704813. |
| title | The abundance of soil fungi with associated functional and taxonomic guilds in pure and mixed beech-conifer tree stands |
| topic | beech-conifer stands; Binary Object; Binary Object (File Size); Binary Object (Media Type); Dassel; Enrichment of European beech forests with conifers; functional guilds; Germany; Goehrde_I; Goehrde_II; Harz; Nienburg; Nienover; OTU abundance; RTG2300; RTG2300-I; soil fungi; Unterluess; VID; Visual identification; Winnefeld |
| url | https://doi.org/10.1594/PANGAEA.933499 |