Seawater carbonate chemistry and bioinformatic quality statistics
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2021
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| _version_ | 1867171877247516672 |
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| author | Durland, Evan de Wit, Pierre Meyer, Eli Langdon, Chris |
| author_facet | Durland, Evan de Wit, Pierre Meyer, Eli Langdon, Chris |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The adaptive capacity of marine calcifiers to ocean acidification (OA) is a topic of great interest to evolutionary biologists and ecologists. Previous studies have provided evidence to suggest that larval resilience to high pCO2 seawater for these species is a trait with a genetic basis and variability in natural populations. To date, however, it remains unclear how the selective effects of OA occur within the context of complex genetic interactions underpinning larval development in many of the most vulnerable taxa. Here we evaluated phenotypic and genetic changes during larval development of Pacific oysters (Crassostrea gigas) reared in ambient (400 µatm) and high (1600 µatm) pCO2 conditions, both in domesticated and naturalized 'wild' oysters from the Pacific Northwest, USA. Using pooled DNA samples, we determined changes in allele frequencies across larval development, from early “D-stage” larvae to metamorphosed juveniles (spat), in both groups and environments. Domesticated larvae had 26% fewer loci with changing allele frequencies across developmental stages and < 50% as many loci affected by acidified culture conditions, compared to larvae from wild brood stock. Functional enrichment analyses of genetic markers with significant changes in allele frequency revealed that the structure and function of cellular membranes were disproportionately affected by high pCO2 conditions in both groups. These results indicate the potential for a rapid adaptive response of oyster populations to OA conditions; however, underlying genetic changes associated with larval development differ between these wild and domesticated oyster stocks and influence their adaptive responses to OA conditions. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_937391 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and bioinformatic quality statistics Durland, Evan de Wit, Pierre Meyer, Eli Langdon, Chris Age; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard error; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Group; Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Percentage; pH, standard error; pH, total scale; Ratio; Reads; Registration number of species; Replicate; Salinity; Salinity, standard error; Single species; Species; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type; Uniform resource locator/link to reference The adaptive capacity of marine calcifiers to ocean acidification (OA) is a topic of great interest to evolutionary biologists and ecologists. Previous studies have provided evidence to suggest that larval resilience to high pCO2 seawater for these species is a trait with a genetic basis and variability in natural populations. To date, however, it remains unclear how the selective effects of OA occur within the context of complex genetic interactions underpinning larval development in many of the most vulnerable taxa. Here we evaluated phenotypic and genetic changes during larval development of Pacific oysters (Crassostrea gigas) reared in ambient (400 µatm) and high (1600 µatm) pCO2 conditions, both in domesticated and naturalized 'wild' oysters from the Pacific Northwest, USA. Using pooled DNA samples, we determined changes in allele frequencies across larval development, from early “D-stage” larvae to metamorphosed juveniles (spat), in both groups and environments. Domesticated larvae had 26% fewer loci with changing allele frequencies across developmental stages and < 50% as many loci affected by acidified culture conditions, compared to larvae from wild brood stock. Functional enrichment analyses of genetic markers with significant changes in allele frequency revealed that the structure and function of cellular membranes were disproportionately affected by high pCO2 conditions in both groups. These results indicate the potential for a rapid adaptive response of oyster populations to OA conditions; however, underlying genetic changes associated with larval development differ between these wild and domesticated oyster stocks and influence their adaptive responses to OA conditions. |
| title | Seawater carbonate chemistry and bioinformatic quality statistics |
| topic | Age; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Aragonite saturation state, standard error; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard error; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Crassostrea gigas; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Group; Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Percentage; pH, standard error; pH, total scale; Ratio; Reads; Registration number of species; Replicate; Salinity; Salinity, standard error; Single species; Species; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.937391 |