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| Main Authors: | , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2021
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.937551 |
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| _version_ | 1867168213379317760 |
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| author | Adam, Nicole Schlicht, Stefanie Han, Yuchen Bechelany, Mikhael Bachmann, Julien Perner, Mirjam |
| author_facet | Adam, Nicole Schlicht, Stefanie Han, Yuchen Bechelany, Mikhael Bachmann, Julien Perner, Mirjam |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogenase activity by means of an activity-based screen. The hydrogen evolution activity of protein extracts from positively screened clones was determined by means of gas chromatography. For the most active clone (SP11F2) the hydrogen evolution activity was determined at different pH conditions and the stability of the respective enzyme ectract under different storage conditions was tested. The datasets contain hydrogen evolution activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_937551 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge Adam, Nicole Schlicht, Stefanie Han, Yuchen Bechelany, Mikhael Bachmann, Julien Perner, Mirjam Accession number, genetics; Area/locality; Comment; Event label; Hydrogenase; Hydrogen evolution activity; Hydrogen evolution activity, standard deviation; hydrothermal vent; M78/2; M78/2_274; M78/2_314; M78/2_324; MARSUED5; Metagenome; Meteor (1986); Remote operated vehicle; ROV; Sample ID; Sample method; Treatment; Treatment: pH; Treatment: temperature; Treatment: time after Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogenase activity by means of an activity-based screen. The hydrogen evolution activity of protein extracts from positively screened clones was determined by means of gas chromatography. For the most active clone (SP11F2) the hydrogen evolution activity was determined at different pH conditions and the stability of the respective enzyme ectract under different storage conditions was tested. The datasets contain hydrogen evolution activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. |
| title | Hydrogen evolution activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge |
| topic | Accession number, genetics; Area/locality; Comment; Event label; Hydrogenase; Hydrogen evolution activity; Hydrogen evolution activity, standard deviation; hydrothermal vent; M78/2; M78/2_274; M78/2_314; M78/2_324; MARSUED5; Metagenome; Meteor (1986); Remote operated vehicle; ROV; Sample ID; Sample method; Treatment; Treatment: pH; Treatment: temperature; Treatment: time after |
| url | https://doi.org/10.1594/PANGAEA.937551 |