Seawater carbonate chemistry and Arsenic accumulation of Crassostrea angulata and Crassostrea gigas
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2018
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| _version_ | 1867171879461060608 |
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| author | Moreira, Anthony Figueira, Etelvina Mestre, Nélia C Schrama, Denise Soares, Amadeu M V M Freitas, Rosa Bebianno, Maria João |
| author_facet | Moreira, Anthony Figueira, Etelvina Mestre, Nélia C Schrama, Denise Soares, Amadeu M V M Freitas, Rosa Bebianno, Maria João |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Proteomic analysis was performed to compare the effects of Arsenic (As), seawater acidification (Low pH) and the combination of both stressors (Low pH + As) on Crassostrea angulata and Crassostrea gigas juveniles in the context of global environmental change. This study aimed to elucidate if two closely related Crassostrea species respond similarly to these environmental stressors, considering both single and combined exposures, to infer if the simultaneous exposure to both stressors induced a differentiated response. Identification of the most important differentially expressed proteins between conditions revealed marked differences in the response of each species towards single and combined exposures, evidencing species-related differences towards each experimental condition. Moreover, protein alterations observed in the combined exposure (Low pH + As) were substantially different from those observed in single exposures. Identified proteins and their putative biological functions revealed an array of modes of action in each condition. Among the most important, those involved in cellular structure (Actin, Atlastin, Severin, Gelsolin, Coronin) and extracellular matrix modulation (Ependymin, Tight junction ZO-1, Neprilysin) were strongly regulated, although in different exposure conditions and species. Data also revealed differences regarding metabolic modulation capacity (ATP beta, Enolase, Aconitate hydratase) and oxidative stress response (Aldehyde dehydrogenase, Lactoylglutathione, Retinal dehydrogenase) of the species, which also depended on single or combined exposures, illustrating a different response capacity of both oyster species to the presence of multiple stressors. Interestingly, alterations of piRNA abundance in C. angulata suggested genome reconfiguration in response to multiple stressors, likely an important mode of action related to adaptive evolution mechanisms previously unknown to oyster species, which requires further investigation. Our findings provide a deeper insight into the complexity of C. angulata and C. gigas responses to environmental stress at the proteome level, evidencing different capacities to endure abiotic changes, with relevance regarding the ecophysiological fitness of the species and competitive advantages in a changing environment. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_943191 |
| institution | PANGAEA |
| language | en |
| publishDate | 2018 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and Arsenic accumulation of Crassostrea angulata and Crassostrea gigas Moreira, Anthony Figueira, Etelvina Mestre, Nélia C Schrama, Denise Soares, Amadeu M V M Freitas, Rosa Bebianno, Maria João Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Arsenic; Arsenic, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Brackish waters; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea angulata; Crassostrea gigas; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Inorganic toxins; Laboratory experiment; Mollusca; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Registration number of species; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference Proteomic analysis was performed to compare the effects of Arsenic (As), seawater acidification (Low pH) and the combination of both stressors (Low pH + As) on Crassostrea angulata and Crassostrea gigas juveniles in the context of global environmental change. This study aimed to elucidate if two closely related Crassostrea species respond similarly to these environmental stressors, considering both single and combined exposures, to infer if the simultaneous exposure to both stressors induced a differentiated response. Identification of the most important differentially expressed proteins between conditions revealed marked differences in the response of each species towards single and combined exposures, evidencing species-related differences towards each experimental condition. Moreover, protein alterations observed in the combined exposure (Low pH + As) were substantially different from those observed in single exposures. Identified proteins and their putative biological functions revealed an array of modes of action in each condition. Among the most important, those involved in cellular structure (Actin, Atlastin, Severin, Gelsolin, Coronin) and extracellular matrix modulation (Ependymin, Tight junction ZO-1, Neprilysin) were strongly regulated, although in different exposure conditions and species. Data also revealed differences regarding metabolic modulation capacity (ATP beta, Enolase, Aconitate hydratase) and oxidative stress response (Aldehyde dehydrogenase, Lactoylglutathione, Retinal dehydrogenase) of the species, which also depended on single or combined exposures, illustrating a different response capacity of both oyster species to the presence of multiple stressors. Interestingly, alterations of piRNA abundance in C. angulata suggested genome reconfiguration in response to multiple stressors, likely an important mode of action related to adaptive evolution mechanisms previously unknown to oyster species, which requires further investigation. Our findings provide a deeper insight into the complexity of C. angulata and C. gigas responses to environmental stress at the proteome level, evidencing different capacities to endure abiotic changes, with relevance regarding the ecophysiological fitness of the species and competitive advantages in a changing environment. |
| title | Seawater carbonate chemistry and Arsenic accumulation of Crassostrea angulata and Crassostrea gigas |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Arsenic; Arsenic, standard deviation; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Brackish waters; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea angulata; Crassostrea gigas; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Inorganic toxins; Laboratory experiment; Mollusca; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Registration number of species; Salinity; Salinity, standard deviation; Single species; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.943191 |