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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2021
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| Acceso en línea: | https://doi.org/10.1594/PANGAEA.943567 |
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| _version_ | 1867168215566647296 |
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| author | Eichner, Meri Wolf-Gladrow, Dieter A Ploug, Helle |
| author_facet | Eichner, Meri Wolf-Gladrow, Dieter A Ploug, Helle |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Photosynthesis and respiration cause distinct chemical microenvironments within cyanobacterial aggregates. Here, we used microsensors and a diffusion–reaction model to characterize gradients in carbonate chemistry and investigate how these are affected by ocean acidification in Baltic vs. Pacific aggregates (Nodularia and Dolichospermum vs. Trichodesmium). Microsensor measurements of O2 and pH were performed under in situ and expected future pCO2 levels on Nodularia and Dolichospermum aggregates collected in the Baltic Sea. Under in situ conditions, O2 and pH levels within the aggregates covered ranges of 80–175% air saturation and 7.7–9.4 in dark and light, respectively. Carbon uptake in the light was predicted to reduce HCO3− by 100–150 μmol/L and CO2 by 3–6 μmol/L in the aggregate center compared to outside, inducing strong CO2 depletion (down to 0.5 μmol/L CO2 remaining in the center) even when assuming that HCO3− covered 80–90% of carbon uptake. Under ocean acidification conditions, enhanced CO2 availability allowed for significantly lower activity of carbon concentrating mechanisms, including a reduction of the contribution of HCO3− to carbon uptake by up to a factor of 10. The magnification of proton gradients under elevated pCO2 that was predicted based on a lower buffer capacity was observed in measurements despite a concurrent decrease in photosynthetic activity. In summary, we provide a quantitative image of the inorganic carbon environment in cyanobacterial aggregates under present-day and expected future conditions, considering both the individual and combined effects of the chemical and biological processes that shape these environments. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_943567 |
| institution | PANGAEA |
| language | en |
| publishDate | 2021 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and carbonate chemistry in the microenvironment within cyanobacterial aggregates Eichner, Meri Wolf-Gladrow, Dieter A Ploug, Helle Acid-base regulation; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Baltic Sea; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Colorimetric; Cyanobacteria; Dolichospermum sp.; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Heterotrophic prokaryotes; Hydrogen ion; Hydrogen ion, standard deviation; Identification; Laboratory experiment; Light mode; Nodularia spumigena; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Oxygen; Oxygen, standard deviation; Oxygen evolution; Oxygen evolution, standard deviation; Oxygen evolution per individual; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Ratio; Registration number of species; Respiration; Salinity; Single species; Species; station_B1; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference Photosynthesis and respiration cause distinct chemical microenvironments within cyanobacterial aggregates. Here, we used microsensors and a diffusion–reaction model to characterize gradients in carbonate chemistry and investigate how these are affected by ocean acidification in Baltic vs. Pacific aggregates (Nodularia and Dolichospermum vs. Trichodesmium). Microsensor measurements of O2 and pH were performed under in situ and expected future pCO2 levels on Nodularia and Dolichospermum aggregates collected in the Baltic Sea. Under in situ conditions, O2 and pH levels within the aggregates covered ranges of 80–175% air saturation and 7.7–9.4 in dark and light, respectively. Carbon uptake in the light was predicted to reduce HCO3− by 100–150 μmol/L and CO2 by 3–6 μmol/L in the aggregate center compared to outside, inducing strong CO2 depletion (down to 0.5 μmol/L CO2 remaining in the center) even when assuming that HCO3− covered 80–90% of carbon uptake. Under ocean acidification conditions, enhanced CO2 availability allowed for significantly lower activity of carbon concentrating mechanisms, including a reduction of the contribution of HCO3− to carbon uptake by up to a factor of 10. The magnification of proton gradients under elevated pCO2 that was predicted based on a lower buffer capacity was observed in measurements despite a concurrent decrease in photosynthetic activity. In summary, we provide a quantitative image of the inorganic carbon environment in cyanobacterial aggregates under present-day and expected future conditions, considering both the individual and combined effects of the chemical and biological processes that shape these environments. |
| title | Seawater carbonate chemistry and carbonate chemistry in the microenvironment within cyanobacterial aggregates |
| topic | Acid-base regulation; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Baltic Sea; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Colorimetric; Cyanobacteria; Dolichospermum sp.; EXP; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Heterotrophic prokaryotes; Hydrogen ion; Hydrogen ion, standard deviation; Identification; Laboratory experiment; Light mode; Nodularia spumigena; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Oxygen; Oxygen, standard deviation; Oxygen evolution; Oxygen evolution, standard deviation; Oxygen evolution per individual; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Ratio; Registration number of species; Respiration; Salinity; Single species; Species; station_B1; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference |
| url | https://doi.org/10.1594/PANGAEA.943567 |