Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas
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| Sprache: | en |
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PANGAEA
2019
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| author | Cao, Ruiwen Zhang, Tianyu Li, Xiao Zhao, Y Wang, Qing Yang, Dinglong Qu, Yi Liu, Hui Zhao, Jianmin |
| author_facet | Cao, Ruiwen Zhang, Tianyu Li, Xiao Zhao, Y Wang, Qing Yang, Dinglong Qu, Yi Liu, Hui Zhao, Jianmin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Ocean acidification (OA) has been found to increase the release of free Cu2+ in seawater. However, only a handful of studies have investigated the influence of OA on Cu accumulation and cellular toxicity in bivalve species. In this study, Pacific oysters, Crassostrea gigas, were exposed to 25 μg/L Cu2+ at three pH levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological parameters [(clearance rate (CR), respiration rate (RR), histopathological damage and condition index (CI)), oxidative stress and neurotoxicity biomarkers [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were investigated in C. gigas. The bioconcentration of Cu was increased in soft tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu could lead to physiological disturbance, oxidative stress, cellular damage, disturbance in energy metabolism and neurotoxicity in oysters. The inhibited CR, increased glycolytic enzymes activities and decreased CI suggested that the energy metabolism strategy adopted by oysters was not sustainable in the long term. Furthermore, integrated biomarker response (IBR) results found that OA and Cu exposure lead to severe stress to oysters, and co-exposure was the most stressful condition. Results from this study highlight the need to include OA in future environmental assessments of pollutants and hazardous materials to better elucidate the risks of those environmental perturbations. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_944162 |
| institution | PANGAEA |
| language | en |
| publishDate | 2019 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas Cao, Ruiwen Zhang, Tianyu Li, Xiao Zhao, Y Wang, Qing Yang, Dinglong Qu, Yi Liu, Hui Zhao, Jianmin Acetylcholinesterase activity, standard deviation; Acetylcholinesterase activity, unit per protein mass; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Clearance rate; Clearance rate, standard deviation; Coast and continental shelf; Condition index; Condition index, standard deviation; Containers and aquaria (20-1000 L or < 1 m**2); Copper; Copper, standard deviation; Crassostrea gigas; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione S-transferase activity, standard deviation; Glutathione S-transferase activity, unit per protein mass; Hexokinase activity, standard deviation; Hexokinase activity, unit per protein mass; Inorganic toxins; Integrated biomarker response index; Laboratory experiment; Lipid peroxidation, per protein mass; Lipid peroxidation, standard deviation; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Pyruvate kinase activity, standard deviation; Pyruvate kinase activity, unit per protein mass; Replicates; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Superoxide dismutase activity, standard deviation; Superoxide dismutase activity, unit per protein mass; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type Ocean acidification (OA) has been found to increase the release of free Cu2+ in seawater. However, only a handful of studies have investigated the influence of OA on Cu accumulation and cellular toxicity in bivalve species. In this study, Pacific oysters, Crassostrea gigas, were exposed to 25 μg/L Cu2+ at three pH levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological parameters [(clearance rate (CR), respiration rate (RR), histopathological damage and condition index (CI)), oxidative stress and neurotoxicity biomarkers [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were investigated in C. gigas. The bioconcentration of Cu was increased in soft tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu could lead to physiological disturbance, oxidative stress, cellular damage, disturbance in energy metabolism and neurotoxicity in oysters. The inhibited CR, increased glycolytic enzymes activities and decreased CI suggested that the energy metabolism strategy adopted by oysters was not sustainable in the long term. Furthermore, integrated biomarker response (IBR) results found that OA and Cu exposure lead to severe stress to oysters, and co-exposure was the most stressful condition. Results from this study highlight the need to include OA in future environmental assessments of pollutants and hazardous materials to better elucidate the risks of those environmental perturbations. |
| title | Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas |
| topic | Acetylcholinesterase activity, standard deviation; Acetylcholinesterase activity, unit per protein mass; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Behaviour; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Clearance rate; Clearance rate, standard deviation; Coast and continental shelf; Condition index; Condition index, standard deviation; Containers and aquaria (20-1000 L or < 1 m**2); Copper; Copper, standard deviation; Crassostrea gigas; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione S-transferase activity, standard deviation; Glutathione S-transferase activity, unit per protein mass; Hexokinase activity, standard deviation; Hexokinase activity, unit per protein mass; Inorganic toxins; Integrated biomarker response index; Laboratory experiment; Lipid peroxidation, per protein mass; Lipid peroxidation, standard deviation; Mollusca; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, standard deviation; pH, total scale; Potentiometric; Potentiometric titration; Pyruvate kinase activity, standard deviation; Pyruvate kinase activity, unit per protein mass; Replicates; Respiration; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Superoxide dismutase activity, standard deviation; Superoxide dismutase activity, unit per protein mass; Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type |
| url | https://doi.org/10.1594/PANGAEA.944162 |