Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2022
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| _version_ | 1867171032786272256 |
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| author | Kwan, Garfield Tsz Tresguerres, Martin |
| author_facet | Kwan, Garfield Tsz Tresguerres, Martin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) exposed to 1600 μatm pCO2 (pH 7.5) were able to fully regulated the pH of both blood and endolymph (the fluid that surrounds the otolith within the inner ear). However, while blood was regulated around pH 7.80, the endolymph was regulated around pH 8.30. These different pH setpoints result in increased pCO2 diffusion into the endolymph, which in turn leads to proportional increases in endolymph [HCO3−] and [CO32−]. Endolymph pH regulation despite the increased pCO2 suggests enhanced H+ removal. However, a lack of differences in inner ear bulk and cell-specific Na+/K+-ATPase and vacuolar type H+-ATPase protein abundance localization pointed out to activation of preexisting ATPases, non-bicarbonate pH buffering, or both, as the mechanism for endolymph pH-regulation. These results provide the first direct evidence showcasing the acid-base chemistry of the endolymph of OA-exposed fish favors otolith overgrowth, and suggests that this phenomenon will be more pronounced in species that count with more robust blood and endolymph pH regulatory mechanisms. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_945127 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish Kwan, Garfield Tsz Tresguerres, Martin Acid-base regulation; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Bicarbonate; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard error; Carbon dioxide, standard error; Chordata; Coast and continental shelf; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hydrogen ion; Hydrogen ion, standard error; Laboratory experiment; Nekton; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Pelagos; pH; pH, standard error; pH, total scale; Potentiometric titration; Proteins; Proteins, standard error; Replicates; Salinity; Salinity, standard error; Sebastes diploproa; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Spectrophotometric; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) exposed to 1600 μatm pCO2 (pH 7.5) were able to fully regulated the pH of both blood and endolymph (the fluid that surrounds the otolith within the inner ear). However, while blood was regulated around pH 7.80, the endolymph was regulated around pH 8.30. These different pH setpoints result in increased pCO2 diffusion into the endolymph, which in turn leads to proportional increases in endolymph [HCO3−] and [CO32−]. Endolymph pH regulation despite the increased pCO2 suggests enhanced H+ removal. However, a lack of differences in inner ear bulk and cell-specific Na+/K+-ATPase and vacuolar type H+-ATPase protein abundance localization pointed out to activation of preexisting ATPases, non-bicarbonate pH buffering, or both, as the mechanism for endolymph pH-regulation. These results provide the first direct evidence showcasing the acid-base chemistry of the endolymph of OA-exposed fish favors otolith overgrowth, and suggests that this phenomenon will be more pronounced in species that count with more robust blood and endolymph pH regulatory mechanisms. |
| title | Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish |
| topic | Acid-base regulation; Alkalinity, total; Alkalinity, total, standard error; Animalia; Aragonite saturation state; Bicarbonate; Bicarbonate ion; Bicarbonate ion, standard error; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Carbonate ion; Carbonate ion, standard error; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard error; Carbon dioxide, standard error; Chordata; Coast and continental shelf; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hydrogen ion; Hydrogen ion, standard error; Laboratory experiment; Nekton; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error; Pelagos; pH; pH, standard error; pH, total scale; Potentiometric titration; Proteins; Proteins, standard error; Replicates; Salinity; Salinity, standard error; Sebastes diploproa; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Spectrophotometric; Temperate; Temperature, water; Temperature, water, standard error; Treatment; Type |
| url | https://doi.org/10.1594/PANGAEA.945127 |