Cuticle elemental concentrations of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification
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| Autores principales: | , , , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2022
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| _version_ | 1867172178053562368 |
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| author | Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer |
| author_facet | Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, inductively-coupled x-ray spectrometry (ICP-MS) was performed on cuticle samples at the Scripps Isotope Geochemistry Laboratory (SIGL) for a precise quantification of elements (µmol/mg sample). The carapace spine was air-dried and then trimmed so only the spine remained with no setae, and the abdominal segment was cut as a 1 x 1 cm² from the center of the second abdominal segment and air-dried. Samples were weighed and placed in Teflon vials for digestion with 0.5 ml of concentrated Teflon-distilled (TD) nitric acid (HNO3) on a hotplate at 100°C for >24 h. Samples were dried down and diluted by a factor of 4000 with 2% TD HNO3 before being transferred to pre-cleaned centrifuge tubes for analysis. Samples were doped with an indium solution to monitor instrumental drift. Measurements were done using a ThermoScientific iCAPq c ICP-MS (Thermo Fisher Scientific GmbH, Bremen, Germany) in standard mode. Masses of Mg and Ca were sequentially measured for 30 ratios, resulting in internal precision of <2% (2 s.d.). Elements were corrected for total mole fraction. Total procedural blanks represented <0.3% of the measurement for Mg and Ca. Raw data were corrected offline for instrument background and drift. Samples were bracketed by internal standards of crab carapace (n=3), which allowed for calculation of absolute values as well as weighted averages of isotopes from natural abundance. The standards yielded external precision of 2% and 3% for Mg and Ca, respectively. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_945342 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Cuticle elemental concentrations of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification Lowder, Kaitlyn deVries, Maya S Hattingh, Ruan Day, James M D Andersson, Andreas J Zerofski, Phillip Taylor, Jennifer Aluminium-27; Barium-137; Body region; Boron-10; Calcium; Calcium-43; Calcium-48; California Current Ecosystem; Chromium-52; Copper-65; crustacean; Identification; Iron-54; Iron-57; La_Jolla_trap_2016; Laboratory experiment; Lead-207(I); Lead-207(II); Lead-207(III); Lead-207(IV); Lead-208; Magnesium; Magnesium-25; Magnesium-26; Ocean acidification; off Southern California; pH, standard deviation; Phosphorus-31; S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Specimen identification; Status; Strontium-86; Tin-119; Titanium-48; Trap, baited; TRAPB; Treatment; Treatment: pH; Treatment: temperature; Type of study; Uranium-238; Weighted average; Zinc-66 We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, inductively-coupled x-ray spectrometry (ICP-MS) was performed on cuticle samples at the Scripps Isotope Geochemistry Laboratory (SIGL) for a precise quantification of elements (µmol/mg sample). The carapace spine was air-dried and then trimmed so only the spine remained with no setae, and the abdominal segment was cut as a 1 x 1 cm² from the center of the second abdominal segment and air-dried. Samples were weighed and placed in Teflon vials for digestion with 0.5 ml of concentrated Teflon-distilled (TD) nitric acid (HNO3) on a hotplate at 100°C for >24 h. Samples were dried down and diluted by a factor of 4000 with 2% TD HNO3 before being transferred to pre-cleaned centrifuge tubes for analysis. Samples were doped with an indium solution to monitor instrumental drift. Measurements were done using a ThermoScientific iCAPq c ICP-MS (Thermo Fisher Scientific GmbH, Bremen, Germany) in standard mode. Masses of Mg and Ca were sequentially measured for 30 ratios, resulting in internal precision of <2% (2 s.d.). Elements were corrected for total mole fraction. Total procedural blanks represented <0.3% of the measurement for Mg and Ca. Raw data were corrected offline for instrument background and drift. Samples were bracketed by internal standards of crab carapace (n=3), which allowed for calculation of absolute values as well as weighted averages of isotopes from natural abundance. The standards yielded external precision of 2% and 3% for Mg and Ca, respectively. |
| title | Cuticle elemental concentrations of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification |
| topic | Aluminium-27; Barium-137; Body region; Boron-10; Calcium; Calcium-43; Calcium-48; California Current Ecosystem; Chromium-52; Copper-65; crustacean; Identification; Iron-54; Iron-57; La_Jolla_trap_2016; Laboratory experiment; Lead-207(I); Lead-207(II); Lead-207(III); Lead-207(IV); Lead-208; Magnesium; Magnesium-25; Magnesium-26; Ocean acidification; off Southern California; pH, standard deviation; Phosphorus-31; S-(Q)-ICP-MS measurements, Thermo Scientific iCAP-Qc ICP-MS; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Specimen identification; Status; Strontium-86; Tin-119; Titanium-48; Trap, baited; TRAPB; Treatment; Treatment: pH; Treatment: temperature; Type of study; Uranium-238; Weighted average; Zinc-66 |
| url | https://doi.org/10.1594/PANGAEA.945342 |