Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2022
|
| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.947112 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867170115032711168 |
|---|---|
| author | Meunier, Cédric Léo Moreno, Hugo Rokitta, Sebastian D Tremblay, Nelly Boersma, Maarten Groß, Elisabeth Klip, Helena Wiltshire, Karen Helen |
| author_facet | Meunier, Cédric Léo Moreno, Hugo Rokitta, Sebastian D Tremblay, Nelly Boersma, Maarten Groß, Elisabeth Klip, Helena Wiltshire, Karen Helen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also quantified the concentration of photosynthetic pigments chlorophyll a (Chl-a) and fucoxanthin. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_947112 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Influence of temperature, pCO2, and dissolved nutrients on the oxidative stress response and the carbon metabolism of phytoplankton Meunier, Cédric Léo Moreno, Hugo Rokitta, Sebastian D Tremblay, Nelly Boersma, Maarten Groß, Elisabeth Klip, Helena Wiltshire, Karen Helen beta-Carotene per cell; Carbon, organic, dissolved exudation, per cell; Carbon dioxide, partial pressure; carbon metabolism; Carbon per cell; Catalase activity, unit per protein mass; Chlorophyll a per cell; Diadinoxanthin per cell; Diatom; Fucoxanthin per cell; global change; Global change vulnerability of North Sea plankton and associated ecosystem services; Glutathione peroxidase activity, unit per protein mass; Glutathione S-transferase activity, unit per protein mass; Growth rate; High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695; Malondialdehyde, per wet mass; Nitrogen/Phosphorus ratio; oxidative stress; Phosphorus, organic, particulate, production per cell; PlanktoSERV; Replicate; Respiration rate, carbon; Strain; Superoxide dismutase manganese activity, unit per protein mass; Temperature, water; TOC analyzer, Shimadzu, TOC-L CPH/CPN; Type of study; Violaxanthin per cell We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also quantified the concentration of photosynthetic pigments chlorophyll a (Chl-a) and fucoxanthin. |
| title | Influence of temperature, pCO2, and dissolved nutrients on the oxidative stress response and the carbon metabolism of phytoplankton |
| topic | beta-Carotene per cell; Carbon, organic, dissolved exudation, per cell; Carbon dioxide, partial pressure; carbon metabolism; Carbon per cell; Catalase activity, unit per protein mass; Chlorophyll a per cell; Diadinoxanthin per cell; Diatom; Fucoxanthin per cell; global change; Global change vulnerability of North Sea plankton and associated ecosystem services; Glutathione peroxidase activity, unit per protein mass; Glutathione S-transferase activity, unit per protein mass; Growth rate; High performance liquid chromatography (HPLC), Agilent, Waters Alliance 2695; Malondialdehyde, per wet mass; Nitrogen/Phosphorus ratio; oxidative stress; Phosphorus, organic, particulate, production per cell; PlanktoSERV; Replicate; Respiration rate, carbon; Strain; Superoxide dismutase manganese activity, unit per protein mass; Temperature, water; TOC analyzer, Shimadzu, TOC-L CPH/CPN; Type of study; Violaxanthin per cell |
| url | https://doi.org/10.1594/PANGAEA.947112 |