Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak (Feb 2020)
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2023
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| author | Rahlff, Janina Esser, Sarah P Heinrichs, Mara Elena Giebel, Helge-Ansgar Probst, Alexander J |
| author_facet | Rahlff, Janina Esser, Sarah P Heinrichs, Mara Elena Giebel, Helge-Ansgar Probst, Alexander J |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_947348 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak (Feb 2020) Rahlff, Janina Esser, Sarah P Heinrichs, Mara Elena Giebel, Helge-Ansgar Probst, Alexander J air-sea; Anemometer, AN-10, Voltcraft; ASSEMBLE_Plus; Association of European Marine Biological Laboratories Expanded; bacterial abundance; Brightness; Comment; DATE/TIME; Date/Time local; EcoMol; Eukaryotes, phototrophic, small; Event label; Flow cytometer, BD Biosciences, BD Accuri C6; Galaxy Sensors smartphone application v.1.8.10; Habitat; LATITUDE; LONGITUDE; Microbial Dispersal from Sea to Air and Snow; MIDSEAS; MULT; Multiple investigations; neuston; Ökologie, Physiologie und Molekularbiologie der Roseobacter-Gruppe: Aufbruch zu einem systembiologischen Verständnis einer global wichtigen Gruppe mariner Bakterien; PhD Research Training Group The Ecology of Molecules; Prokaryotes; Salinity; Sample method; sea foam; sea-surface microlayer; Skagerrak; Skagerrak_1; Skagerrak_10a; Skagerrak_10b; Skagerrak_11; Skagerrak_12/R; Skagerrak_2; Skagerrak_3; Skagerrak_4; Skagerrak_5; Skagerrak_6; Skagerrak_7; Skagerrak_8; Skagerrak_9; Station label; Temperature, water; Thermosalinometer, portable, WTW Multiline 3420; TRR51; virus-like particles; Virus-like particles; Wind speed, maximum; Wind speed, minimum Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022. |
| title | Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak (Feb 2020) |
| topic | air-sea; Anemometer, AN-10, Voltcraft; ASSEMBLE_Plus; Association of European Marine Biological Laboratories Expanded; bacterial abundance; Brightness; Comment; DATE/TIME; Date/Time local; EcoMol; Eukaryotes, phototrophic, small; Event label; Flow cytometer, BD Biosciences, BD Accuri C6; Galaxy Sensors smartphone application v.1.8.10; Habitat; LATITUDE; LONGITUDE; Microbial Dispersal from Sea to Air and Snow; MIDSEAS; MULT; Multiple investigations; neuston; Ökologie, Physiologie und Molekularbiologie der Roseobacter-Gruppe: Aufbruch zu einem systembiologischen Verständnis einer global wichtigen Gruppe mariner Bakterien; PhD Research Training Group The Ecology of Molecules; Prokaryotes; Salinity; Sample method; sea foam; sea-surface microlayer; Skagerrak; Skagerrak_1; Skagerrak_10a; Skagerrak_10b; Skagerrak_11; Skagerrak_12/R; Skagerrak_2; Skagerrak_3; Skagerrak_4; Skagerrak_5; Skagerrak_6; Skagerrak_7; Skagerrak_8; Skagerrak_9; Station label; Temperature, water; Thermosalinometer, portable, WTW Multiline 3420; TRR51; virus-like particles; Virus-like particles; Wind speed, maximum; Wind speed, minimum |
| url | https://doi.org/10.1594/PANGAEA.947348 |