Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2022
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| _version_ | 1867170409147793408 |
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| author | Kammann, Sandra Karsten, Ulf Glaser, Karin Schiefelbein, Ulf Hassenrück, Christiane Mikhailyuk, Tatiana Demchenko, Eduardo Dolnik, Christian Leinweber, Peter |
| author_facet | Kammann, Sandra Karsten, Ulf Glaser, Karin Schiefelbein, Ulf Hassenrück, Christiane Mikhailyuk, Tatiana Demchenko, Eduardo Dolnik, Christian Leinweber, Peter |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Nomarski DIC optics (Olympus Ltd, Hamburg, Germany). Micrographs were taken with a digital camera (Olympus LC30) attached to the microscope, and processed by the Olympus software cellSens Entry. Direct microscopy of rewetted samples was performed in parallel with cultivation for evaluation of dominating species of algae and cyanobacteria in the original samples. Morphological identification of the biocrust organisms was based mainly on Ettl and Gärtner (2014) for green microalgae, and on Komárek (2013) for cyanobacteria, as well as on some monographs and papers devoted to taxonomic revisions of the taxa of interest (Darienko and Pröschold 2019). Moss and lichens samples were air-dried after collection. For determination, a microscope with a maximum magnification of 400x was used. Morphological identification of mosses followed Frahm and Frey (2004) with taxonomical reference to (Hodgetts et al. 2020). Lichens were determined according to Wirth et al. (2013). Morphologically critical species of the genus Cladonia where additionally analyzed by thin-layer chromatography according to (Culberson and Ammann 1979) in solvent system A. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_947829 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany Kammann, Sandra Karsten, Ulf Glaser, Karin Schiefelbein, Ulf Hassenrück, Christiane Mikhailyuk, Tatiana Demchenko, Eduardo Dolnik, Christian Leinweber, Peter 16S rRNA; Actinochloris terrestris; algae; Amandinea punctata; Asterococcus sp.; Bacidina etayana; Brachythecium albicans; Bracteacoccus sp.; Bryum capillare; Caloplaca cerinella; Campylopus introflexus; Cephaloziella divaricata; Ceratodon purpureus; Chlorella vulgaris; Chlorococcum sp.; Chloroidium cf. ellipsoideum; Chlorokybus atmophyticus; Chlorolobion sp.; Cladonia arbuscula; Cladonia chlorophaea; Cladonia ciliata; Cladonia coccifera; Cladonia conista; Cladonia fimbriata; Cladonia floerkeana; Cladonia foliacea; Cladonia furcata; Cladonia glauca; Cladonia gracilis; Cladonia humilis; Cladonia macilenta; Cladonia novochlorophaea; Cladonia phyllophora; Cladonia portentosa; Cladonia ramulosa; Cladonia rangiformis; Cladonia rei; Cladonia scabriuscula; Cladonia subulata; Cladonia uncialis ssp. biuncialis; Coccomyxa sp.; Coelastrella sp.; Crusts; Cylindrocystis cf. crassa; Cylindrocystis sp.; Darßer Ort, Mecklenburg Western Pomerania, Germany; DATE/TIME; Dicranum scoparium; Diplosphaera chodatii; DOBD; DOeGD; DOlGD; DOWD; dune; Elevation of event; Elliptochloris subsphaerica; Eremochloris sp.; Event label; Evernia prunastri; Field experiment; Hennediella heimii; Hypnum cupressiforme; Hypnum cupressiforme var. lacunosum; Hypnum jutlandicum; Hypogymnia physodes; Interfilum cf. massjukiae; Interfilum terricola; Klebsormidium cf. flaccidum; Klebsormidium cf. subtile; Klebsormidium crenulatum; Latitude of event; Lecania cyrtella; Lecanora hagenii; Lecanora persimilis; Leptolyngbya sp.; Light microscope; Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics; Lobochlamys sp.; Location; Longitude of event; Lophozia bicuspidata; Macrochloris sp.; Micarea misella; Microcoleus vaginatus; Myrmecia cf. irregularis; Nannochloris sp.; Nodosilinea cf. epilithica; Nostoc cf. commune; Nostoc cf. edaphicum; Nostoc cf. linckia; Nostoc sp.; Oxyrrhynchium praelongum; Parietochloris cf. alveolaris; Parmelia sulcata; Peltigera extenuata; Peltigera hymenia; Physcia tenella; Placynthiella uliginosa; Planophila sp.; Pleurozium schreberi; POBD; POGD; Pohlia nutans; Polytrichum juniperinum; Polytrichum piliferum; POWD; POWW; Pramort, Mecklenburg Western Pomerania, Germany; PreDCI; PreIS; Prerow, Mecklenburg Western Pomerania, Germany; ProGD; ProH; Pseudochlorella sp.; Pseudomuriella cf. aurantiaca; Pseudoscleropodium purum; Ptilidium ciliatum; Ptychostomum capillare; Ptychostomum compactum; Ptychostomum imbricatulum; Ptychostomum moravicum; Racomitrium canescens; Racomitrium elongatum; Rügen, Mecklenburg Western Pomerania, Germany; SchBD; SchGD; SchPD; SchWD; Scoliciosporum gallurae; sediment analysis; Site; soil ecology; Spongiochloris sp.; Stenomitos sp.; Stichococcus allas; Stichococcus cf. bacillaris; Syntrichia ruraliformis; Tetracystis cf. sarcinalis; Tetradesmus arenicola; Thin layer chromatography; Timaviella sp.; Tolypothrix cf. byssoidea; VerctF; Verden (Aller), Lower Saxony, Germany; VerTsZ; Watanabea cf. acidophila; Xanthoria parietina This dataset comprises the microbial community composition of biological soil crusts in north-German sand dunes. For this we obtained enrichment cultures of phototrophic microorganisms, by placing fragments of biocrusts of the same Petri dishes as used for sequencing, in Petri dishes with Bold Basal (1N BBM) agarized medium (Bischoff and Bold 1963). Cultures were grown under standard laboratory conditions: with a 12-hour alteration of light and dark phases and irradiation of 25 μmol photons m-2 s-1 at a temperature 20 ± 5 ºС. Microscopic study of these raw cultures began in the third week of cultivation. Morphological examinations were performed using Olympus BX53 light microscope with Nomarski DIC optics (Olympus Ltd, Hamburg, Germany). Micrographs were taken with a digital camera (Olympus LC30) attached to the microscope, and processed by the Olympus software cellSens Entry. Direct microscopy of rewetted samples was performed in parallel with cultivation for evaluation of dominating species of algae and cyanobacteria in the original samples. Morphological identification of the biocrust organisms was based mainly on Ettl and Gärtner (2014) for green microalgae, and on Komárek (2013) for cyanobacteria, as well as on some monographs and papers devoted to taxonomic revisions of the taxa of interest (Darienko and Pröschold 2019). Moss and lichens samples were air-dried after collection. For determination, a microscope with a maximum magnification of 400x was used. Morphological identification of mosses followed Frahm and Frey (2004) with taxonomical reference to (Hodgetts et al. 2020). Lichens were determined according to Wirth et al. (2013). Morphologically critical species of the genus Cladonia where additionally analyzed by thin-layer chromatography according to (Culberson and Ammann 1979) in solvent system A. |
| title | Morphological determination of the phototrophic community composition of biological soil crusts in coastal sand dunes in northern Germany |
| topic | 16S rRNA; Actinochloris terrestris; algae; Amandinea punctata; Asterococcus sp.; Bacidina etayana; Brachythecium albicans; Bracteacoccus sp.; Bryum capillare; Caloplaca cerinella; Campylopus introflexus; Cephaloziella divaricata; Ceratodon purpureus; Chlorella vulgaris; Chlorococcum sp.; Chloroidium cf. ellipsoideum; Chlorokybus atmophyticus; Chlorolobion sp.; Cladonia arbuscula; Cladonia chlorophaea; Cladonia ciliata; Cladonia coccifera; Cladonia conista; Cladonia fimbriata; Cladonia floerkeana; Cladonia foliacea; Cladonia furcata; Cladonia glauca; Cladonia gracilis; Cladonia humilis; Cladonia macilenta; Cladonia novochlorophaea; Cladonia phyllophora; Cladonia portentosa; Cladonia ramulosa; Cladonia rangiformis; Cladonia rei; Cladonia scabriuscula; Cladonia subulata; Cladonia uncialis ssp. biuncialis; Coccomyxa sp.; Coelastrella sp.; Crusts; Cylindrocystis cf. crassa; Cylindrocystis sp.; Darßer Ort, Mecklenburg Western Pomerania, Germany; DATE/TIME; Dicranum scoparium; Diplosphaera chodatii; DOBD; DOeGD; DOlGD; DOWD; dune; Elevation of event; Elliptochloris subsphaerica; Eremochloris sp.; Event label; Evernia prunastri; Field experiment; Hennediella heimii; Hypnum cupressiforme; Hypnum cupressiforme var. lacunosum; Hypnum jutlandicum; Hypogymnia physodes; Interfilum cf. massjukiae; Interfilum terricola; Klebsormidium cf. flaccidum; Klebsormidium cf. subtile; Klebsormidium crenulatum; Latitude of event; Lecania cyrtella; Lecanora hagenii; Lecanora persimilis; Leptolyngbya sp.; Light microscope; Light microscope, Olympus Ltd, BX53, with Nomarski DIC optics; Lobochlamys sp.; Location; Longitude of event; Lophozia bicuspidata; Macrochloris sp.; Micarea misella; Microcoleus vaginatus; Myrmecia cf. irregularis; Nannochloris sp.; Nodosilinea cf. epilithica; Nostoc cf. commune; Nostoc cf. edaphicum; Nostoc cf. linckia; Nostoc sp.; Oxyrrhynchium praelongum; Parietochloris cf. alveolaris; Parmelia sulcata; Peltigera extenuata; Peltigera hymenia; Physcia tenella; Placynthiella uliginosa; Planophila sp.; Pleurozium schreberi; POBD; POGD; Pohlia nutans; Polytrichum juniperinum; Polytrichum piliferum; POWD; POWW; Pramort, Mecklenburg Western Pomerania, Germany; PreDCI; PreIS; Prerow, Mecklenburg Western Pomerania, Germany; ProGD; ProH; Pseudochlorella sp.; Pseudomuriella cf. aurantiaca; Pseudoscleropodium purum; Ptilidium ciliatum; Ptychostomum capillare; Ptychostomum compactum; Ptychostomum imbricatulum; Ptychostomum moravicum; Racomitrium canescens; Racomitrium elongatum; Rügen, Mecklenburg Western Pomerania, Germany; SchBD; SchGD; SchPD; SchWD; Scoliciosporum gallurae; sediment analysis; Site; soil ecology; Spongiochloris sp.; Stenomitos sp.; Stichococcus allas; Stichococcus cf. bacillaris; Syntrichia ruraliformis; Tetracystis cf. sarcinalis; Tetradesmus arenicola; Thin layer chromatography; Timaviella sp.; Tolypothrix cf. byssoidea; VerctF; Verden (Aller), Lower Saxony, Germany; VerTsZ; Watanabea cf. acidophila; Xanthoria parietina |
| url | https://doi.org/10.1594/PANGAEA.947829 |