Isochrysis sp.'s responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm)
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| Main Authors: | , , , , , , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2022
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| author | Rokitta, Sebastian D Großmann, Christian H Werner, Elisa Moye, Jannika Castellani, Giulia Nöthig, Eva-Maria Rost, Björn |
| author_facet | Rokitta, Sebastian D Großmann, Christian H Werner, Elisa Moye, Jannika Castellani, Giulia Nöthig, Eva-Maria Rost, Björn |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We assessed the responses of solitary cells of Arctic Isochrysis sp. (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_948489 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Isochrysis sp.'s responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) Rokitta, Sebastian D Großmann, Christian H Werner, Elisa Moye, Jannika Castellani, Giulia Nöthig, Eva-Maria Rost, Björn Alkalinity, total; Bottle incubation; calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006); calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic nitrogen (PON) quota; calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota; Carbon, inorganic, dissolved; Carbon dioxide, partial pressure; Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001); Coulter counter, Beckman Coulter, Multisizer 3; DATE/TIME; Electron transport rate, relative; Elemental analyzer, EuroVector, EuroEA; EXP; Experiment; Experimental treatment; Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016); Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201; Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996); Fram Strait; Identification; Initial slope of the photosynthesis-irradiance curve; Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light; Isochrysis_sp_PS78; Isochrysis sp.; Isochrysis sp., carbon, organic, particulate/nitrogen, organic, particulate ratio; Isochrysis sp., chlorophyll a/carbon, organic, particulate ratio; Isochrysis sp., chlorophyll a quota per cell; Isochrysis sp., growth rate; Isochrysis sp., particulate organic carbon production per cell; Isochrysis sp., particulate organic carbon quota per cell; Isochrysis sp., particulate organic nitrogen production per cell; Isochrysis sp., particulate organic nitrogen quota per cell; Light; Light acclimation index; Maximum photosynthesis rate, oxygen, per chlorophyll a; model simulation; pCO2; pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system; pH, NBS scale; pH 826 mobile handheld device, with Aquatrode Plus, Metrohm; Phytoplankton; RCP8.5; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Temperature; Temperature, water; Thermometer, internal, Aquatrode Plus, Metrohm; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR We assessed the responses of solitary cells of Arctic Isochrysis sp. (Strain PS78) grown under a matrix of temperature (2°C vs. 6°C), light intensity (55 vs. 160 μmol photons m-2 s-1) and CO2 partial pressures (pCO2; 400 vs. 1000 μatm). Before the experiments, the strain (isolated during Polarstern cruise PS78 in 2011) was kept as stock culture at 1° in 0.2 µm sterile-filtered Arctic seawater (Salinity 33), enriched with vitamins and trace metals according to F/2 medium (Guillard & Ryther, 1962). Nitrate and phosphate were added in concentrations of 100 and 6 µmol L-1, respectively. Experiments were conducted between May 2016 and September 2017 at the Alfred-Wegener-Institute, using standardized media and continuous light exposition. Next to acclimation parameters (growth rates, particulate and dissolved organic carbon and nitrogen, chlorophyll a content), we measured physiological processes in-vivo (electron transport rates and net photosynthesis) using fast-repetition rate fluorometry and membrane-inlet mass spectrometry. |
| title | Isochrysis sp.'s responses to temperature (2 vs. 6 °C), light (55 vs. 160 µmol photons m-2 s-1) and pCO2 (400 vs. 1000 µatm) |
| topic | Alkalinity, total; Bottle incubation; calculated from carbonate chemistry using the CO2Sys Excel sheet (Pierrot, Lewis & Wallace, 2006); calculated from chlorophyll a (chl a) and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic carbon (POC) quota; calculated from growth rate and particulate organic nitrogen (PON) quota; calculated from particulate organic carbon (POC) and particulate organic nitrogen (PON) quota; Carbon, inorganic, dissolved; Carbon dioxide, partial pressure; Colorimetric detection, TRAACs continuous flow autoanalyzer, according to the method of Stoll et al. (2001); Coulter counter, Beckman Coulter, Multisizer 3; DATE/TIME; Electron transport rate, relative; Elemental analyzer, EuroVector, EuroEA; EXP; Experiment; Experimental treatment; Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012), raw data obtained using a membrane-inlet mass spectrometer (MIMS) as described in Kottmeier, Rokitta & Rost (2016); Fitted parameter using the photosynthesis vs. Irradiance equation from Rokitta & Rost (2012); raw data obtained using a fast-repetition rate fluoremeter (FRRF), FastOcean PTX with FastAct Laboratory system, Chelsea Technologies after Oxborough et al. (201; Fluorometer, Turner Designs, TD-700, using acidification method (Knap et al., 1996); Fram Strait; Identification; Initial slope of the photosynthesis-irradiance curve; Initial slope of the photosynthesis-irradiance curve, relative electron transfer rate per unit light; Isochrysis_sp_PS78; Isochrysis sp.; Isochrysis sp., carbon, organic, particulate/nitrogen, organic, particulate ratio; Isochrysis sp., chlorophyll a/carbon, organic, particulate ratio; Isochrysis sp., chlorophyll a quota per cell; Isochrysis sp., growth rate; Isochrysis sp., particulate organic carbon production per cell; Isochrysis sp., particulate organic carbon quota per cell; Isochrysis sp., particulate organic nitrogen production per cell; Isochrysis sp., particulate organic nitrogen quota per cell; Light; Light acclimation index; Maximum photosynthesis rate, oxygen, per chlorophyll a; model simulation; pCO2; pCO2 mixed from CO2-free air and pure CO2 with a custom built gas mixing system; pH, NBS scale; pH 826 mobile handheld device, with Aquatrode Plus, Metrohm; Phytoplankton; RCP8.5; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Temperature; Temperature, water; Thermometer, internal, Aquatrode Plus, Metrohm; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Universal light meter & data logger, WALZ, ULM-500, with 4Pi sensor, LI-COR |
| url | https://doi.org/10.1594/PANGAEA.948489 |