Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry
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| Main Authors: | , , , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2022
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| _version_ | 1867170115885203456 |
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| author | Venn, Alexander A Tambutté, Eric Steeve, Comeau Tambutté, Sylvie |
| author_facet | Venn, Alexander A Tambutté, Eric Steeve, Comeau Tambutté, Sylvie |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Internal pH measurements made in the extracellular calcifying medium (ECM), calcifying (calicoblastic) epithelium and mesoglea of the coral Stylophora pistillata using the fluorescent dye SNARF-1 and confocal microscopy. The measurements were made in light and darkness three experiments. Experiment 1 involved using coral samples maintained at pH 8 seawater. Experiment 2 involved placing samples in 4 seawater acidification conditions: pH 8, 7.8, 7.4 and 7.2 for 1 week. Experiment 3 involved placing samples in 4 levels of dissolved inorganic carbon concentration: elevated; ambient, low and very low for one week. The research was carried out at the Centre Scientifique de Monaco between 2014-2017. The aim of the experiment was to determine the pH gradient across the calcifying cell layer and determine how it responded to the three experiments. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_949400 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry Venn, Alexander A Tambutté, Eric Steeve, Comeau Tambutté, Sylvie biomineralization; Climate change; Comment; Confocal microscope, Leica, SP5; EXP; Experiment; Experiment/study setup; Laboratory experiment; Laboratory-experiments; pH; pH, extracellular; pH, extracellular, standard deviation; pH, intracellular; pH, intracellular, standard deviation; pH, mesoglea; pH, mesoglea, standard deviation; pH, standard deviation; pH regulation; physiology; Salinity; scleractinians; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Thermometer; Treatment Internal pH measurements made in the extracellular calcifying medium (ECM), calcifying (calicoblastic) epithelium and mesoglea of the coral Stylophora pistillata using the fluorescent dye SNARF-1 and confocal microscopy. The measurements were made in light and darkness three experiments. Experiment 1 involved using coral samples maintained at pH 8 seawater. Experiment 2 involved placing samples in 4 seawater acidification conditions: pH 8, 7.8, 7.4 and 7.2 for 1 week. Experiment 3 involved placing samples in 4 levels of dissolved inorganic carbon concentration: elevated; ambient, low and very low for one week. The research was carried out at the Centre Scientifique de Monaco between 2014-2017. The aim of the experiment was to determine the pH gradient across the calcifying cell layer and determine how it responded to the three experiments. |
| title | Proton gradients across the coral calcifying cell layer: effects of light, ocean acidification and carbonate chemistry |
| topic | biomineralization; Climate change; Comment; Confocal microscope, Leica, SP5; EXP; Experiment; Experiment/study setup; Laboratory experiment; Laboratory-experiments; pH; pH, extracellular; pH, extracellular, standard deviation; pH, intracellular; pH, intracellular, standard deviation; pH, mesoglea; pH, mesoglea, standard deviation; pH, standard deviation; pH regulation; physiology; Salinity; scleractinians; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Thermometer; Treatment |
| url | https://doi.org/10.1594/PANGAEA.949400 |