Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2022
|
| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.949824 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867171034936901632 |
|---|---|
| author | Gutekunst, Cordula Nina Liebner, Susanne Jenner, Anna-Kathrina Knorr, Klaus-Holger Unger, Viktoria Koebsch, Franziska Racasa, Erwin Don Yang, Sizhong Böttcher, Michael Ernst Janssen, Manon Kallmeyer, Jens Otto, Denise Schmiedinger, Iris Winski, Lucas Jurasinski, Gerald |
| author_facet | Gutekunst, Cordula Nina Liebner, Susanne Jenner, Anna-Kathrina Knorr, Klaus-Holger Unger, Viktoria Koebsch, Franziska Racasa, Erwin Don Yang, Sizhong Böttcher, Michael Ernst Janssen, Manon Kallmeyer, Jens Otto, Denise Schmiedinger, Iris Winski, Lucas Jurasinski, Gerald |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The rewetting of peatlands is a promising measure to mitigate greenhouse gas (GHG) emissions by preventing the further mineralization of the peat soil through aeration. In coastal peatland, the rewetting with brackish water can increase the GHG mitigation potential by the introduction of sulfate, a terminal electron acceptor (TEA). Sulfate is known to lower the CH4 production and thus, its emission by favoring the growth of sulfate-reducers, which outcompete methanogens for substrate. The data contain porewater variables such as pH, electrical conductivity (EC) and sulfate, chloride, dissolved CO2 and CH4 concentrations, as well as absolute abundances of methane- and sulfate-cycling microbial communities. The data were collected in spring and autumn 2019 after a storm surge with brackish water inflow in January 2019. Field sampling was conducted in the nature reserve Heiligensee and Hütelmoor in North-East Germany, close to the Southern Baltic Sea coast. We took peat cores using a Russian peat corer in addition to pore water diffusion samplers and plastic liners (length: 60cm; inner diameter 10 cm) at four locations along a transect from further inland towards the Baltic Sea. We wanted to compare the soil and pore water geochemistry as well as the microbial communities after the brackish water inflow to the common freshwater rewetting state. Pore water was extracted using pore water suction samplers in the lab and environmental variables were quantified with an ICP. Microbial samples were sampled from the peat core using sterile equipment. We used quantitative polymerase chain reaction (qPCR) to characterize pools of DNA and cDNA targeting total and putatively active bacteria and archaea. qPCR was performed on key functional genes of methane production (mcrA), aerobic methane oxidation (pmoA) and sulfate reduction (dsrB) in addition to the 16S rRNA gene for the absolute abundance of total prokaryotes. Furthermore, we retrieved soil plugs to determine the concentrations and isotopic signatures of dissolved trace gases (CO2/DIC and CH4) in the pore water. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_949824 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Effects of a brackish water inflow on peat pore water variables and methane-cycling microbial community abundances in a freshwater rewetted coastal fen Gutekunst, Cordula Nina Liebner, Susanne Jenner, Anna-Kathrina Knorr, Klaus-Holger Unger, Viktoria Koebsch, Franziska Racasa, Erwin Don Yang, Sizhong Böttcher, Michael Ernst Janssen, Manon Kallmeyer, Jens Otto, Denise Schmiedinger, Iris Winski, Lucas Jurasinski, Gerald Carbon dioxide; Cavity ring-down spectroscopy; Chloride; Coastal peatland rewetting; Conductivity, electrical; CRDS; Date/Time of event; Depth, relative; DEPTH, soil; Depth, soil, maximum; Depth, soil, minimum; Device type; Event label; GASC; Gas chromatograph; Gene copies per gram sediment; Ion coupled plasma spectrometry (ICP); Latitude of event; Longitude of event; Methane; methanogens; methanotrophs; Northeastern Germany; pH; pH meter; pore water geochemistry; Real-time quantitative polymerase chain reaction (qPCR); Refractometer; Replicate; RPC; Russian peat corer; Sample comment; Sample ID; Sulfate; sulfate-reducing bacteria; TRANSCOAST_Hutelmoor_HC1; TRANSCOAST_Hutelmoor_HC2-1; TRANSCOAST_Hutelmoor_HC2-2; TRANSCOAST_Hutelmoor_HC3; TRANSCOAST_Hutelmoor_HC4; Type of study; δ13C, dissolved inorganic carbon; δ13C, methane The rewetting of peatlands is a promising measure to mitigate greenhouse gas (GHG) emissions by preventing the further mineralization of the peat soil through aeration. In coastal peatland, the rewetting with brackish water can increase the GHG mitigation potential by the introduction of sulfate, a terminal electron acceptor (TEA). Sulfate is known to lower the CH4 production and thus, its emission by favoring the growth of sulfate-reducers, which outcompete methanogens for substrate. The data contain porewater variables such as pH, electrical conductivity (EC) and sulfate, chloride, dissolved CO2 and CH4 concentrations, as well as absolute abundances of methane- and sulfate-cycling microbial communities. The data were collected in spring and autumn 2019 after a storm surge with brackish water inflow in January 2019. Field sampling was conducted in the nature reserve Heiligensee and Hütelmoor in North-East Germany, close to the Southern Baltic Sea coast. We took peat cores using a Russian peat corer in addition to pore water diffusion samplers and plastic liners (length: 60cm; inner diameter 10 cm) at four locations along a transect from further inland towards the Baltic Sea. We wanted to compare the soil and pore water geochemistry as well as the microbial communities after the brackish water inflow to the common freshwater rewetting state. Pore water was extracted using pore water suction samplers in the lab and environmental variables were quantified with an ICP. Microbial samples were sampled from the peat core using sterile equipment. We used quantitative polymerase chain reaction (qPCR) to characterize pools of DNA and cDNA targeting total and putatively active bacteria and archaea. qPCR was performed on key functional genes of methane production (mcrA), aerobic methane oxidation (pmoA) and sulfate reduction (dsrB) in addition to the 16S rRNA gene for the absolute abundance of total prokaryotes. Furthermore, we retrieved soil plugs to determine the concentrations and isotopic signatures of dissolved trace gases (CO2/DIC and CH4) in the pore water. |
| title | Effects of a brackish water inflow on peat pore water variables and methane-cycling microbial community abundances in a freshwater rewetted coastal fen |
| topic | Carbon dioxide; Cavity ring-down spectroscopy; Chloride; Coastal peatland rewetting; Conductivity, electrical; CRDS; Date/Time of event; Depth, relative; DEPTH, soil; Depth, soil, maximum; Depth, soil, minimum; Device type; Event label; GASC; Gas chromatograph; Gene copies per gram sediment; Ion coupled plasma spectrometry (ICP); Latitude of event; Longitude of event; Methane; methanogens; methanotrophs; Northeastern Germany; pH; pH meter; pore water geochemistry; Real-time quantitative polymerase chain reaction (qPCR); Refractometer; Replicate; RPC; Russian peat corer; Sample comment; Sample ID; Sulfate; sulfate-reducing bacteria; TRANSCOAST_Hutelmoor_HC1; TRANSCOAST_Hutelmoor_HC2-1; TRANSCOAST_Hutelmoor_HC2-2; TRANSCOAST_Hutelmoor_HC3; TRANSCOAST_Hutelmoor_HC4; Type of study; δ13C, dissolved inorganic carbon; δ13C, methane |
| url | https://doi.org/10.1594/PANGAEA.949824 |