Seawater carbonate chemistry in experiment 1 and 2
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2022
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| author | Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo |
| author_facet | Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/pCO2. In two laboratory experiments, we tested the effect of acidification, as well as pH/pCO2 variability, on gene expression in the brain tissue of a common temperate kelp forest/estuarine fish, Embiotoca jacksoni. Experiment 1 employed static pH treatments (target pH = 7.85/7.30), while Experiment 2 incorporated two variable treatments that oscillated around corresponding static treatments with the same mean (target pH = 7.85/7.70) in an eight-day cycle (amplitude ± 0.15). We found that patterns of global gene expression differed across pH level treatments. Additionally, we identified differential expression of specific genes and enrichment of specific gene sets (GSEA) in comparisons of static pH treatments and in comparisons of static and variable pH treatments of the same mean pH. Importantly, we found that pH/pCO2 variability decreased the number of differentially expressed genes detected between high and low pH treatments, and that inter-individual variability in gene expression was greater in variable treatments than static treatments. These results provide important confirmation of neurological impacts of acidification in a temperate fish species and, critically, that natural environmental variability may mediate the impacts of ocean acidification. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_951362 |
| institution | PANGAEA |
| language | en |
| publishDate | 2022 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry in experiment 1 and 2 Toy, Jason A Kroeker, Kristy J Logan, Cheryl A Takeshita, Yuichiro Longo, Gary C Bernardi, Giacomo Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Embiotoca jacksoni; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/pCO2. In two laboratory experiments, we tested the effect of acidification, as well as pH/pCO2 variability, on gene expression in the brain tissue of a common temperate kelp forest/estuarine fish, Embiotoca jacksoni. Experiment 1 employed static pH treatments (target pH = 7.85/7.30), while Experiment 2 incorporated two variable treatments that oscillated around corresponding static treatments with the same mean (target pH = 7.85/7.70) in an eight-day cycle (amplitude ± 0.15). We found that patterns of global gene expression differed across pH level treatments. Additionally, we identified differential expression of specific genes and enrichment of specific gene sets (GSEA) in comparisons of static pH treatments and in comparisons of static and variable pH treatments of the same mean pH. Importantly, we found that pH/pCO2 variability decreased the number of differentially expressed genes detected between high and low pH treatments, and that inter-individual variability in gene expression was greater in variable treatments than static treatments. These results provide important confirmation of neurological impacts of acidification in a temperate fish species and, critically, that natural environmental variability may mediate the impacts of ocean acidification. |
| title | Seawater carbonate chemistry in experiment 1 and 2 |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Calculated using seacarb after Orr et al. (2018); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Embiotoca jacksoni; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Fugacity of carbon dioxide in seawater, standard deviation; Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type |
| url | https://doi.org/10.1594/PANGAEA.951362 |