Prokaryotic community (mayor clades contributing >0.1% of the total 16S rRNA sequences) in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019)
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| Autores principales: | , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2023
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| _version_ | 1867171882801823744 |
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| author | Pajares, Silvia Merino, Martin Farías, Laura |
| author_facet | Pajares, Silvia Merino, Martin Farías, Laura |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Seawater samples were collected with 10-L Niskin bottles in four stations along a cross-shore transect in front of the Port of Mazatlán (Mexico) during the oceanographic cruises Maz IV (April 2018) and Maz V (April 2019) on board the R/V “El Puma” (UNAM). Samples for molecular analyses (16S rRNA sequences) were collected at different depths based on the oxygen and chlorophyll-a profiles: surface (5 m depth); deep chlorophyll maximum (at 10–45 m depth, except in the coastal station S1 of Maz V where a sample was collected at the mixed layer); base of the oxycline (at 60–125 m depth); OMZ core (only for Maz IV at 250 m depth); and bottom (between 23 m depth in S1 and 670 m depth in S4). Water samples were filtered on board through polycarbonate membranes (0.22-μm pore, Merck Millipore). Genomic DNA was extracted with the DNeasy PowerWater Kit (Qiagen). The V3–V4 region of the 16S rRNA gene was amplified using the index and adaptor-linked primers Bakt_341F and Bakt_805R, and then sequenced using a 2 × 300 bp paired-end Illumina Miseq system. Amplicon libraries were analyzed with the software QIIME 2. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_954089 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Prokaryotic community (mayor clades contributing >0.1% of the total 16S rRNA sequences) in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019) Pajares, Silvia Merino, Martin Farías, Laura Campaign; Clade; CTD, Sea-Bird, SBE 19plus; Date/Time of event; Depth, description; El Nino Southern Oscillation; El Puma; Event label; Latitude of event; Longitude of event; Maz_IV; Maz_IV_S1; Maz_IV_S2; Maz_IV_S3; Maz_IV_S4; Maz_V; Maz_V_S1; Maz_V_S2; Maz_V_S3; Maz_V_S4; Mexican Pacific; MiSeq sequencer (DNA sequencer); nitrogen genes; Oxygen minimum zone; Port of Mazatlán; prokaryotic community; Sample code/label; Sequence abundance; Station label Seawater samples were collected with 10-L Niskin bottles in four stations along a cross-shore transect in front of the Port of Mazatlán (Mexico) during the oceanographic cruises Maz IV (April 2018) and Maz V (April 2019) on board the R/V “El Puma” (UNAM). Samples for molecular analyses (16S rRNA sequences) were collected at different depths based on the oxygen and chlorophyll-a profiles: surface (5 m depth); deep chlorophyll maximum (at 10–45 m depth, except in the coastal station S1 of Maz V where a sample was collected at the mixed layer); base of the oxycline (at 60–125 m depth); OMZ core (only for Maz IV at 250 m depth); and bottom (between 23 m depth in S1 and 670 m depth in S4). Water samples were filtered on board through polycarbonate membranes (0.22-μm pore, Merck Millipore). Genomic DNA was extracted with the DNeasy PowerWater Kit (Qiagen). The V3–V4 region of the 16S rRNA gene was amplified using the index and adaptor-linked primers Bakt_341F and Bakt_805R, and then sequenced using a 2 × 300 bp paired-end Illumina Miseq system. Amplicon libraries were analyzed with the software QIIME 2. |
| title | Prokaryotic community (mayor clades contributing >0.1% of the total 16S rRNA sequences) in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019) |
| topic | Campaign; Clade; CTD, Sea-Bird, SBE 19plus; Date/Time of event; Depth, description; El Nino Southern Oscillation; El Puma; Event label; Latitude of event; Longitude of event; Maz_IV; Maz_IV_S1; Maz_IV_S2; Maz_IV_S3; Maz_IV_S4; Maz_V; Maz_V_S1; Maz_V_S2; Maz_V_S3; Maz_V_S4; Mexican Pacific; MiSeq sequencer (DNA sequencer); nitrogen genes; Oxygen minimum zone; Port of Mazatlán; prokaryotic community; Sample code/label; Sequence abundance; Station label |
| url | https://doi.org/10.1594/PANGAEA.954089 |