Abundance of N-cycling genes in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019)
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2023
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| _version_ | 1867170117656248320 |
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| author | Pajares, Silvia Merino, Martin Farías, Laura |
| author_facet | Pajares, Silvia Merino, Martin Farías, Laura |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Seawater samples were collected with 10-L Niskin bottles in four stations along a cross-shore transect in front of the Port of Mazatlán (Mexico) during the oceanographic cruises Maz IV (April 2018) and Maz V (April 2019) on board the R/V "El Puma" (UNAM). Samples for molecular analyses (abundance of N-cycling genes) were collected at different depths based on the oxygen and chlorophyll-a profiles: surface (5 m depth); mixed layer, deep chlorophyll maximum (at 10–45 m depth); oxycline, base of the oxycline (at 60–125 m depth); upper OMZ (only for Maz V); OMZ core (at 250 m depth); and bottom (between 23 m depth in S1 and 670 m depth in S4). Water samples were filtered on board through polycarbonate membranes (0.22-μm pore, Merck Millipore). Genomic DNA was extracted with the DNeasy PowerWater Kit (Qiagen). The abundance of N-cycling genes was assessed using the gene copy number of bacterial and archaeal amoA, anammox bacterial 16S rRNA, nirK, nirS, and nrfA, using a StepOnePlus real-time PCR system (Applied Biosystems). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_954155 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Abundance of N-cycling genes in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019) Pajares, Silvia Merino, Martin Farías, Laura Campaign; CTD, Sea-Bird, SBE 19plus; Date/Time of event; Depth, description; DEPTH, water; Elevation of event; El Nino Southern Oscillation; El Puma; Event label; Gene copies per volume; Gene name; Latitude of event 2; Longitude of event; Maz_IV; Maz_IV_S1; Maz_IV_S2; Maz_IV_S3; Maz_IV_S4; Maz_V; Maz_V_S1; Maz_V_S2; Maz_V_S3; Maz_V_S4; Mexican Pacific; nitrogen genes; Oxygen minimum zone; Port of Mazatlán; prokaryotic community; Real-Time PCR, Applied Biosystems, StepOnePlus; Sample code/label; Station label Seawater samples were collected with 10-L Niskin bottles in four stations along a cross-shore transect in front of the Port of Mazatlán (Mexico) during the oceanographic cruises Maz IV (April 2018) and Maz V (April 2019) on board the R/V "El Puma" (UNAM). Samples for molecular analyses (abundance of N-cycling genes) were collected at different depths based on the oxygen and chlorophyll-a profiles: surface (5 m depth); mixed layer, deep chlorophyll maximum (at 10–45 m depth); oxycline, base of the oxycline (at 60–125 m depth); upper OMZ (only for Maz V); OMZ core (at 250 m depth); and bottom (between 23 m depth in S1 and 670 m depth in S4). Water samples were filtered on board through polycarbonate membranes (0.22-μm pore, Merck Millipore). Genomic DNA was extracted with the DNeasy PowerWater Kit (Qiagen). The abundance of N-cycling genes was assessed using the gene copy number of bacterial and archaeal amoA, anammox bacterial 16S rRNA, nirK, nirS, and nrfA, using a StepOnePlus real-time PCR system (Applied Biosystems). |
| title | Abundance of N-cycling genes in an oxygen-deficient upwelling system during La Niña (2018) and El Niño (2019) |
| topic | Campaign; CTD, Sea-Bird, SBE 19plus; Date/Time of event; Depth, description; DEPTH, water; Elevation of event; El Nino Southern Oscillation; El Puma; Event label; Gene copies per volume; Gene name; Latitude of event 2; Longitude of event; Maz_IV; Maz_IV_S1; Maz_IV_S2; Maz_IV_S3; Maz_IV_S4; Maz_V; Maz_V_S1; Maz_V_S2; Maz_V_S3; Maz_V_S4; Mexican Pacific; nitrogen genes; Oxygen minimum zone; Port of Mazatlán; prokaryotic community; Real-Time PCR, Applied Biosystems, StepOnePlus; Sample code/label; Station label |
| url | https://doi.org/10.1594/PANGAEA.954155 |