Seawater carbonate chemistry and cellular inorganic carbon fluxes in Trichodesmium
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2015
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| _version_ | 1867171883509612544 |
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| author | Eichner, Meri Thoms, Silke Kranz, Sven A Rost, Björn |
| author_facet | Eichner, Meri Thoms, Silke Kranz, Sven A Rost, Björn |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | To predict effects of climate change on phytoplankton, it is crucial to understand how their mechanisms for carbon acquisition respond to environmental conditions. Aiming to shed light on the responses of extra- and intracellular inorganic C (Ci) fluxes, the cyanobacterium Trichodesmium erythraeum IMS101 was grown with different nitrogen sources (N2 vs NO3 –) and pCO2 levels (380 vs 1400 µatm). Cellular Ci fluxes were assessed by combining membrane inlet mass spectrometry (MIMS), 13C fractionation measurements, and modelling. Aside from a significant decrease in Ci affinity at elevated pCO2 and changes in CO2 efflux with different N sources, extracellular Ci fluxes estimated by MIMS were largely unaffected by the treatments. 13C fractionation during biomass production, however, increased with pCO2, irrespective of the N source. Strong discrepancies were observed in CO2 leakage estimates obtained by MIMS and a 13C-based approach, which further increased under elevated pCO2. These offsets could be explained by applying a model that comprises extracellular CO2 and HCO3– fluxes as well as internal Ci cycling around the carboxysome via the CO2 uptake facilitator NDH-14. Assuming unidirectional, kinetic fractionation between CO2 and HCO3– in the cytosol or enzymatic fractionation by NDH-14, both significantly improved the comparability of leakage estimates. Our results highlight the importance of internal Ci cycling for 13C composition as well as cellular energy budgets of Trichodesmium, which ought to be considered in process studies on climate change effects. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_956021 |
| institution | PANGAEA |
| language | en |
| publishDate | 2015 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and cellular inorganic carbon fluxes in Trichodesmium Eichner, Meri Thoms, Silke Kranz, Sven A Rost, Björn Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bicarbonate uptake in chlorophyll, standard deviation; Bicarbonate uptake rate, per chlorophyll a; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide efflux, per chlorophyll a; Carbon dioxide efflux, per chlorophyll a, standard deviation; Carbon dioxide uptake, per chlorophyll, standard deviation; Carbon dioxide uptake rate, per chlorophyll a; Carbon uptake rate, standard deviation; Cyanobacteria; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross carbon uptake rate, per chlorophyll a; Laboratory experiment; Laboratory strains; Net carbon fixation rate, per chlorophyll a; Net carbon fixation rate, standard deviation; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phytoplankton; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Salinity; Single species; Species; Temperature, water; Time point, descriptive; Treatment; Trichodesmium erythraeum; Type To predict effects of climate change on phytoplankton, it is crucial to understand how their mechanisms for carbon acquisition respond to environmental conditions. Aiming to shed light on the responses of extra- and intracellular inorganic C (Ci) fluxes, the cyanobacterium Trichodesmium erythraeum IMS101 was grown with different nitrogen sources (N2 vs NO3 –) and pCO2 levels (380 vs 1400 µatm). Cellular Ci fluxes were assessed by combining membrane inlet mass spectrometry (MIMS), 13C fractionation measurements, and modelling. Aside from a significant decrease in Ci affinity at elevated pCO2 and changes in CO2 efflux with different N sources, extracellular Ci fluxes estimated by MIMS were largely unaffected by the treatments. 13C fractionation during biomass production, however, increased with pCO2, irrespective of the N source. Strong discrepancies were observed in CO2 leakage estimates obtained by MIMS and a 13C-based approach, which further increased under elevated pCO2. These offsets could be explained by applying a model that comprises extracellular CO2 and HCO3– fluxes as well as internal Ci cycling around the carboxysome via the CO2 uptake facilitator NDH-14. Assuming unidirectional, kinetic fractionation between CO2 and HCO3– in the cytosol or enzymatic fractionation by NDH-14, both significantly improved the comparability of leakage estimates. Our results highlight the importance of internal Ci cycling for 13C composition as well as cellular energy budgets of Trichodesmium, which ought to be considered in process studies on climate change effects. |
| title | Seawater carbonate chemistry and cellular inorganic carbon fluxes in Trichodesmium |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bicarbonate uptake in chlorophyll, standard deviation; Bicarbonate uptake rate, per chlorophyll a; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide efflux, per chlorophyll a; Carbon dioxide efflux, per chlorophyll a, standard deviation; Carbon dioxide uptake, per chlorophyll, standard deviation; Carbon dioxide uptake rate, per chlorophyll a; Carbon uptake rate, standard deviation; Cyanobacteria; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gross carbon uptake rate, per chlorophyll a; Laboratory experiment; Laboratory strains; Net carbon fixation rate, per chlorophyll a; Net carbon fixation rate, standard deviation; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, NBS scale; pH, standard deviation; pH, total scale; Phytoplankton; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Salinity; Single species; Species; Temperature, water; Time point, descriptive; Treatment; Trichodesmium erythraeum; Type |
| url | https://doi.org/10.1594/PANGAEA.956021 |