Seawater carbonate chemistry and larval viability, size, development, and shell mineralization of the eastern oyster (Crassostrea virginica)
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2023
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| _version_ | 1867170118940753920 |
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| author | Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem |
| author_facet | Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Calcifying marine organisms, including the eastern oyster (Crassostrea virginica), are vulnerable to ocean acidification (OA) because it is more difficult to precipitate calcium carbonate (CaCO3). Previous investigations of the molecular mechanisms associated with resilience to OA in C. virginica demonstrated significant differences in single nucleotide polymorphism and gene expression profiles among oysters reared under ambient and OA conditions. Converged evidence generated by both of these approaches highlighted the role of genes related to biomineralization, including perlucins. Here, gene silencing via RNA interference (RNAi) was used to evaluate the protective role of a perlucin gene under OA stress. Larvae were exposed to short dicer-substrate small interfering RNA (DsiRNA-perlucin) to silence the target gene or to one of two control treatments (control DsiRNA or seawater) before cultivation under OA (pH ~7.3) or ambient (pH ~8.2) conditions. Two transfection experiments were performed in parallel, one during fertilization and one during early larval development (6 h post-fertilization), before larval viability, size, development, and shell mineralization were monitored. Silenced oysters under acidification stress were the smallest, had shell abnormalities, and had significantly reduced shell mineralization, thereby suggesting that perlucin significantly helps larvae mitigate the effects of OA. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_957629 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and larval viability, size, development, and shell mineralization of the eastern oyster (Crassostrea virginica) Schwaner, Caroline Espinosa, Emmanuelle Pales Allam, Bassem Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Birefringence intensity; Birefringence intensity, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Crassostrea virginica; Day of experiment; Development; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression, fold change, relative; Gene expression, fold change, relative, standard deviation; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Laboratory strains; Larval deformity rate; Larval deformity rate, standard deviation; Length; Length, standard deviation; Mollusca; Mortality/Survival; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Salinity; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Treatment; Treatment: pH; Type of study; Viability; Viability, standard deviation; Zooplankton Calcifying marine organisms, including the eastern oyster (Crassostrea virginica), are vulnerable to ocean acidification (OA) because it is more difficult to precipitate calcium carbonate (CaCO3). Previous investigations of the molecular mechanisms associated with resilience to OA in C. virginica demonstrated significant differences in single nucleotide polymorphism and gene expression profiles among oysters reared under ambient and OA conditions. Converged evidence generated by both of these approaches highlighted the role of genes related to biomineralization, including perlucins. Here, gene silencing via RNA interference (RNAi) was used to evaluate the protective role of a perlucin gene under OA stress. Larvae were exposed to short dicer-substrate small interfering RNA (DsiRNA-perlucin) to silence the target gene or to one of two control treatments (control DsiRNA or seawater) before cultivation under OA (pH ~7.3) or ambient (pH ~8.2) conditions. Two transfection experiments were performed in parallel, one during fertilization and one during early larval development (6 h post-fertilization), before larval viability, size, development, and shell mineralization were monitored. Silenced oysters under acidification stress were the smallest, had shell abnormalities, and had significantly reduced shell mineralization, thereby suggesting that perlucin significantly helps larvae mitigate the effects of OA. |
| title | Seawater carbonate chemistry and larval viability, size, development, and shell mineralization of the eastern oyster (Crassostrea virginica) |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Birefringence intensity; Birefringence intensity, standard deviation; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Crassostrea virginica; Day of experiment; Development; Experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression, fold change, relative; Gene expression, fold change, relative, standard deviation; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Laboratory strains; Larval deformity rate; Larval deformity rate, standard deviation; Length; Length, standard deviation; Mollusca; Mortality/Survival; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Salinity; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperature, water; Treatment; Treatment: pH; Type of study; Viability; Viability, standard deviation; Zooplankton |
| url | https://doi.org/10.1594/PANGAEA.957629 |