Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: measurements of cell size and density
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2023
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| _version_ | 1867170417718853632 |
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| author | Matthiessen, Birte Hattich, Giannina S I Pulina, Silvia Hansen, Thomas Reusch, Thorsten B H Hamer, Jorin |
| author_facet | Matthiessen, Birte Hattich, Giannina S I Pulina, Silvia Hansen, Thomas Reusch, Thorsten B H Hamer, Jorin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Measurements of cell size, cell density, and nutrient concentration in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. Cell density and cell volume were measured at the end of each batch cycle for both species. Phosphate, Nitrate and Nitrite were measured at the end of batch cycles 1, 7, 13, and 26. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_957873 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: measurements of cell size and density Matthiessen, Birte Hattich, Giannina S I Pulina, Silvia Hansen, Thomas Reusch, Thorsten B H Hamer, Jorin Batch; Biovolume; Bottle number; cell size; Chaetoceros affinis; Chaetoceros affinis, biovolume; Chaetoceros affinis, cell biovolume; Chaetoceros affinis, diameter; Chaetoceros affinis, height; Day of experiment; diatoms; Emiliania huxleyi; Emiliania huxleyi, biovolume; Emiliania huxleyi, cell biovolume; Emiliania huxleyi, diameter; evolution; Flow cytometer, Beckman Coulter, Gallios; Genotype Sorting; Laboratory experiment; Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1; Morphotype; Nitrate; Nitrite; nutrients; phenotypic plasticity; Phosphate; Phytoplankton; Priority Programme 1704 DynaTrait: Flexibility Matters: Interplay between Trait Diversity and Ecological Dynamics Using Aquatic Communities as Model Systems; Sampling date/time, experiment; SEAL Analytical, continuous flow analyser QuAAtro; Species Sorting; SPP1704; trait variability; Treatment: nutrients; Type of study Measurements of cell size, cell density, and nutrient concentration in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. Cell density and cell volume were measured at the end of each batch cycle for both species. Phosphate, Nitrate and Nitrite were measured at the end of batch cycles 1, 7, 13, and 26. |
| title | Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: measurements of cell size and density |
| topic | Batch; Biovolume; Bottle number; cell size; Chaetoceros affinis; Chaetoceros affinis, biovolume; Chaetoceros affinis, cell biovolume; Chaetoceros affinis, diameter; Chaetoceros affinis, height; Day of experiment; diatoms; Emiliania huxleyi; Emiliania huxleyi, biovolume; Emiliania huxleyi, cell biovolume; Emiliania huxleyi, diameter; evolution; Flow cytometer, Beckman Coulter, Gallios; Genotype Sorting; Laboratory experiment; Microscopy, Zeiss, Axiovert 200 and Axio Observer Z1; Morphotype; Nitrate; Nitrite; nutrients; phenotypic plasticity; Phosphate; Phytoplankton; Priority Programme 1704 DynaTrait: Flexibility Matters: Interplay between Trait Diversity and Ecological Dynamics Using Aquatic Communities as Model Systems; Sampling date/time, experiment; SEAL Analytical, continuous flow analyser QuAAtro; Species Sorting; SPP1704; trait variability; Treatment: nutrients; Type of study |
| url | https://doi.org/10.1594/PANGAEA.957873 |