Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: genotyping
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2023
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| _version_ | 1867170417791205376 |
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| author | Matthiessen, Birte Hattich, Giannina S I Pulina, Silvia Hansen, Thomas Reusch, Thorsten B H Hamer, Jorin |
| author_facet | Matthiessen, Birte Hattich, Giannina S I Pulina, Silvia Hansen, Thomas Reusch, Thorsten B H Hamer, Jorin |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Measurements of genotype composition in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. The genotype composition was assessed for each species after batch cycles 7,13, and 26 by using microsatellites. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_957916 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: genotyping Matthiessen, Birte Hattich, Giannina S I Pulina, Silvia Hansen, Thomas Reusch, Thorsten B H Hamer, Jorin Batch; Bottle number; cell size; diatoms; evolution; Genotype; Genotype Sorting; Genotyping using microsatellites; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Priority Programme 1704 DynaTrait: Flexibility Matters: Interplay between Trait Diversity and Ecological Dynamics Using Aquatic Communities as Model Systems; Sample ID; Sampling date/time, experiment; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Species Sorting; SPP1704; trait variability; Treatment: nutrients; Type of study Measurements of genotype composition in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes (http://roscoff-culture-collection.org/; Emiliania huxleyi: GC22: RCC6383, C42: RCC6376, C41: RCC6375, C47: RCC4546, C96: RCC6381, C91: RCC6380, C35: RCC6374, C30: RCC6371, C48: RCC6377, Chaetoceros affinis: B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350). The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply. Nutrient concentrations were added as 9.0 ± 0.7 (10 N), 19.6 ± 0.8 (20 N), or 29.9 ± 0.9 μmoL-1 (30 N) nitrate and 0.9 ± 0.1 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 4:1 N:Si ratio. Cultivation was done in a semi-continuous batch cycle system with transfer of 2.88e7 ± 1.57e7 µm³ biovolume into a new batch every 7 days. Prior to the experimental start, genotypes were separately acclimated to experimental conditions with 20 μmol L−1 nitrate for 7 days. The genotype composition was assessed for each species after batch cycles 7,13, and 26 by using microsatellites. |
| title | Long-term experiment (182 days) with the phytoplankton species Chaetoceros affinis and Emiliania huxleyi at three nutrient regimes: genotyping |
| topic | Batch; Bottle number; cell size; diatoms; evolution; Genotype; Genotype Sorting; Genotyping using microsatellites; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Priority Programme 1704 DynaTrait: Flexibility Matters: Interplay between Trait Diversity and Ecological Dynamics Using Aquatic Communities as Model Systems; Sample ID; Sampling date/time, experiment; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Species Sorting; SPP1704; trait variability; Treatment: nutrients; Type of study |
| url | https://doi.org/10.1594/PANGAEA.957916 |