Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Dataset Open Access |
| Language: | en |
| Published: |
PANGAEA
2023
|
| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.958972 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1867172345081233408 |
|---|---|
| author | Dang, Xin Lee, Tin Hang Thiyagarajan, Vengatesen |
| author_facet | Dang, Xin Lee, Tin Hang Thiyagarajan, Vengatesen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The carbon dioxide induced ocean acidification (OA) process is well known to have profound effects on physiology, survival and immune responses in marine organisms, and particularly calcifiers including edible oysters. At the same time, some wild populations could develop a complex and sophisticated immune system to cope with multiple biotic and abiotic stresses, such as bacterial infections and OA, over the long period of coevolution with the environment. However, it is unclear how immunological responses and the underlying mechanisms are altered under the combined effect of OA and bacterial infection, especially in the ecologically and economically important edible oysters. Here, we collected the wild population of oyster species Crassostrea hongkongensis (the Hong Kong oyster) from their native estuarine area and carried out a bacterial challenge with the worldwide pervasive pathogen of human foodborne disease, Vibrio parahaemolyticus, to investigate the host immune responses and molecular mechanisms under the high-CO2 and low pH-driven OA conditions. The wild population had a high immune resistance to OA, but the resistance is compromised under the combined effect of OA and bacterial infection both in vivo or in vitro. We classified all transcriptomic genes based on expression profiles and functional pathways and identified the specifically switched on and off genes and pathways under combined effect. These genes and pathways were mainly involved in multiple immunological processes including pathogen recognition, immune signal transduction and effectors. This work would help understand how the immunological function and mechanism response to bacterial infection in wild populations and predict the dynamic distribution of human health-related pathogens to reduce the risk of foodborne disease under the future climate change scenario. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_958972 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and wild oyster population resistance to ocean acidification Dang, Xin Lee, Tin Hang Thiyagarajan, Vengatesen Alkalinity, total; Animalia; Apoptosis cells; Aragonite saturation state; Bacteria clearance in hemocytes; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea hongkongensis; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hand picking; Hong Kong, Region of the People's Republic of China; Immunology/Self-protection; Laboratory experiment; Mollusca; Mortality/Survival; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other; Pak_Nai_Field; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, total scale; Potentiometric; Potentiometric titration; Replicate; Salinity; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Survival; Temperature, water; Time in hours; Treatment; Tropical; Type of study The carbon dioxide induced ocean acidification (OA) process is well known to have profound effects on physiology, survival and immune responses in marine organisms, and particularly calcifiers including edible oysters. At the same time, some wild populations could develop a complex and sophisticated immune system to cope with multiple biotic and abiotic stresses, such as bacterial infections and OA, over the long period of coevolution with the environment. However, it is unclear how immunological responses and the underlying mechanisms are altered under the combined effect of OA and bacterial infection, especially in the ecologically and economically important edible oysters. Here, we collected the wild population of oyster species Crassostrea hongkongensis (the Hong Kong oyster) from their native estuarine area and carried out a bacterial challenge with the worldwide pervasive pathogen of human foodborne disease, Vibrio parahaemolyticus, to investigate the host immune responses and molecular mechanisms under the high-CO2 and low pH-driven OA conditions. The wild population had a high immune resistance to OA, but the resistance is compromised under the combined effect of OA and bacterial infection both in vivo or in vitro. We classified all transcriptomic genes based on expression profiles and functional pathways and identified the specifically switched on and off genes and pathways under combined effect. These genes and pathways were mainly involved in multiple immunological processes including pathogen recognition, immune signal transduction and effectors. This work would help understand how the immunological function and mechanism response to bacterial infection in wild populations and predict the dynamic distribution of human health-related pathogens to reduce the risk of foodborne disease under the future climate change scenario. |
| title | Seawater carbonate chemistry and wild oyster population resistance to ocean acidification |
| topic | Alkalinity, total; Animalia; Apoptosis cells; Aragonite saturation state; Bacteria clearance in hemocytes; Benthic animals; Benthos; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea hongkongensis; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Hand picking; Hong Kong, Region of the People's Republic of China; Immunology/Self-protection; Laboratory experiment; Mollusca; Mortality/Survival; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other; Pak_Nai_Field; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, NBS scale; pH, total scale; Potentiometric; Potentiometric titration; Replicate; Salinity; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Survival; Temperature, water; Time in hours; Treatment; Tropical; Type of study |
| url | https://doi.org/10.1594/PANGAEA.958972 |