Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109
Fuente:
PANGAEA
Enregistré dans:
| Auteurs principaux: | , , |
|---|---|
| Format: | Dataset Open Access |
| Langue: | en |
| Publié: |
PANGAEA
2024
|
| Sujets: | |
| Accès en ligne: | |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
| _version_ | 1867168222151704576 |
|---|---|
| author | Bodur, Yasemin V Felden, Janine Braeckman, Ulrike |
| author_facet | Bodur, Yasemin V Felden, Janine Braeckman, Ulrike |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_959552 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109 Bodur, Yasemin V Felden, Janine Braeckman, Ulrike Acridine Orange Direct Counting (AODC); AODC; ARK-XXXI/4; B_LANDER; Bottom lander; Counts; Date/Time of event; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Elevation of event; Estimated from counts according to Hobbie et al. (1977); Event label; Filter; FRAM; FRontiers in Arctic marine Monitoring; Latitude of event; Longitude of event; Method/Device of event; Microbial abundance, cells; Multicorer with television; Number of cells; Polarstern; PS109; PS109_105-2; PS109_115-2; PS109_122-1; PS109_125-3; PS109_139-2; PS109_139-3; PS109_154-1; PS109_19-4; PS109_36-2; PS109_45-4; PS109_61-1; PS109_62-1; PS109_68-1; PS109_69-1; PS109_76-2; PS109_93-2; Sample number; Standard deviation; TVMUC Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany). |
| title | Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109 |
| topic | Acridine Orange Direct Counting (AODC); AODC; ARK-XXXI/4; B_LANDER; Bottom lander; Counts; Date/Time of event; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Elevation of event; Estimated from counts according to Hobbie et al. (1977); Event label; Filter; FRAM; FRontiers in Arctic marine Monitoring; Latitude of event; Longitude of event; Method/Device of event; Microbial abundance, cells; Multicorer with television; Number of cells; Polarstern; PS109; PS109_105-2; PS109_115-2; PS109_122-1; PS109_125-3; PS109_139-2; PS109_139-3; PS109_154-1; PS109_19-4; PS109_36-2; PS109_45-4; PS109_61-1; PS109_62-1; PS109_68-1; PS109_69-1; PS109_76-2; PS109_93-2; Sample number; Standard deviation; TVMUC |
| url | https://doi.org/10.1594/PANGAEA.959552 |