Inorganic ion concentrations in bodyfluids of marine invertebrates exposed to low salinity in the laboratory
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| Autores principales: | , , , , , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2023
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| _version_ | 1867171327021940736 |
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| author | Podbielski, Imke Anna Hiebenthal, Claas Hajati, Mithra-Christin Bock, Christian Bleich, Markus Melzner, Frank |
| author_facet | Podbielski, Imke Anna Hiebenthal, Claas Hajati, Mithra-Christin Bock, Christian Bleich, Markus Melzner, Frank |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Laboratory experiments were conducted in the climate chambers at GEOMAR Helmholtz Centre for Ocean Research Kiel in the time between March and November 2018. Experiments were designed to study the effect of long-term (1 month) exposure to low salinity in osmoconforming invertebrates. The study organisms (Asterias rubens, Mytilus edulis, Littorina littorea, Diadumene lineata, Strongylocentrotus droebachiensis and Psammechinus milliaris) were collected in Kiel Fjord, Eckernförder Bight or the Kattegat from spring to autumn 2018. Organisms were acclimated to climate chamber conditions for 1 week (under habitat salinity, 14˚C, constant aeration) and then subjected to salinity acclimation for 1-2 weeks until the final salinity treatment level was reached. Then different salinity treatments were maintained for 4 weeks. Water physiochemistry (temperature, salinity, pH, nitrite, nitrate, phosphate) was recorded frequently. After the experiment, samples were taken from seawater and body fluids to measure total osmolality (mosmol/kg) with an osmomat and inorganic ions (mmol/l). No body fluid samples were taken from Diadumene lineata as organisms were too small and volumes too low. Anions were measured with a novel protocol via ion chromatography, cations were measured via flame photometry. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_959683 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Inorganic ion concentrations in bodyfluids of marine invertebrates exposed to low salinity in the laboratory Podbielski, Imke Anna Hiebenthal, Claas Hajati, Mithra-Christin Bock, Christian Bleich, Markus Melzner, Frank Asterias_rubens_2018; Baltic Sea, Eckernförde Bay; Baltic Sea, Kattegat; BD; Bromide; Calcium ion; Calculated average/mean values; cellular volume regulation; Chloride; Climate change; Date/time end, experiment; Date/time start, experiment; Diadumene_lineata_2018; Dredge, benthos; Event label; Flame photometry; Fluoride; Freeze-depression osmometer, Gonotec, Osmomat 030; HAND; Individual code; invertebrates; Ion chromatography; Kieler Bucht; Laboratory experiment; Littorina_littorea_2018; Medium; Mytilus_edulis_2018; Nitrate; osmoconformer; Osmolality; osmolytes; osmoregulation; Phosphate; Potassium ion; Psammechinus_miliaris_2018; salinity tolerance; Sampling by hand; Sodium ion; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strongylocentrotus_droebachiensis_2018; Sulfate; Tank number; Temperature, water; Treatment: salinity; Type of study; VID; Visual identification Laboratory experiments were conducted in the climate chambers at GEOMAR Helmholtz Centre for Ocean Research Kiel in the time between March and November 2018. Experiments were designed to study the effect of long-term (1 month) exposure to low salinity in osmoconforming invertebrates. The study organisms (Asterias rubens, Mytilus edulis, Littorina littorea, Diadumene lineata, Strongylocentrotus droebachiensis and Psammechinus milliaris) were collected in Kiel Fjord, Eckernförder Bight or the Kattegat from spring to autumn 2018. Organisms were acclimated to climate chamber conditions for 1 week (under habitat salinity, 14˚C, constant aeration) and then subjected to salinity acclimation for 1-2 weeks until the final salinity treatment level was reached. Then different salinity treatments were maintained for 4 weeks. Water physiochemistry (temperature, salinity, pH, nitrite, nitrate, phosphate) was recorded frequently. After the experiment, samples were taken from seawater and body fluids to measure total osmolality (mosmol/kg) with an osmomat and inorganic ions (mmol/l). No body fluid samples were taken from Diadumene lineata as organisms were too small and volumes too low. Anions were measured with a novel protocol via ion chromatography, cations were measured via flame photometry. |
| title | Inorganic ion concentrations in bodyfluids of marine invertebrates exposed to low salinity in the laboratory |
| topic | Asterias_rubens_2018; Baltic Sea, Eckernförde Bay; Baltic Sea, Kattegat; BD; Bromide; Calcium ion; Calculated average/mean values; cellular volume regulation; Chloride; Climate change; Date/time end, experiment; Date/time start, experiment; Diadumene_lineata_2018; Dredge, benthos; Event label; Flame photometry; Fluoride; Freeze-depression osmometer, Gonotec, Osmomat 030; HAND; Individual code; invertebrates; Ion chromatography; Kieler Bucht; Laboratory experiment; Littorina_littorea_2018; Medium; Mytilus_edulis_2018; Nitrate; osmoconformer; Osmolality; osmolytes; osmoregulation; Phosphate; Potassium ion; Psammechinus_miliaris_2018; salinity tolerance; Sampling by hand; Sodium ion; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strongylocentrotus_droebachiensis_2018; Sulfate; Tank number; Temperature, water; Treatment: salinity; Type of study; VID; Visual identification |
| url | https://doi.org/10.1594/PANGAEA.959683 |