Lipid contents in sinking diatom aggregates incubated in rotating pressure and control tanks
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| Autores principales: | , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2023
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| _version_ | 1867171327466536960 |
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| author | Becker, Kevin W Elvert, Marcus |
| author_facet | Becker, Kevin W Elvert, Marcus |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level. Volumetric contents of diatom-specific lipids in sinking diatom aggregates were determined by High Performance Liquid Chromatography (HPLC) coupled to quadrupole Time-of-Flight Mass Spectrometry (qToF-MS) in samples retrieved every 4 days throughout the 20-day incubation experiment. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_960466 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Lipid contents in sinking diatom aggregates incubated in rotating pressure and control tanks Becker, Kevin W Elvert, Marcus biological carbon pump; Bruker maXis UHR TOF mass spectrometer coupled to a Dionex Ultimate 3000 UHPLC; Date/time end, experiment; Date/time start, experiment; Deep sea; Diatom; Digital manometer, Keller AG, LEO5; Experiment; Experimental treatment; HADAL_aggregates; Hadal trench; hydrostatic pressure; Laboratory; Laboratory experiment; lipids; marine carbon cycle; marine snow; microbial community; Monogalactosyldiacylglycerol; Monogalactosyldiacylglycerol, standard deviation; Pigments; Plastoquinone-9:9; Plastoquinone-9:9, standard deviation; Replicate; Respiration; Treatment: pressure; Treatment: time after; Triacylglycerol, standard deviation; Triacylglycerols; Type of study; Ubiquinone-10:10; Ubiquinone-10:10, standard deviation The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level. Volumetric contents of diatom-specific lipids in sinking diatom aggregates were determined by High Performance Liquid Chromatography (HPLC) coupled to quadrupole Time-of-Flight Mass Spectrometry (qToF-MS) in samples retrieved every 4 days throughout the 20-day incubation experiment. |
| title | Lipid contents in sinking diatom aggregates incubated in rotating pressure and control tanks |
| topic | biological carbon pump; Bruker maXis UHR TOF mass spectrometer coupled to a Dionex Ultimate 3000 UHPLC; Date/time end, experiment; Date/time start, experiment; Deep sea; Diatom; Digital manometer, Keller AG, LEO5; Experiment; Experimental treatment; HADAL_aggregates; Hadal trench; hydrostatic pressure; Laboratory; Laboratory experiment; lipids; marine carbon cycle; marine snow; microbial community; Monogalactosyldiacylglycerol; Monogalactosyldiacylglycerol, standard deviation; Pigments; Plastoquinone-9:9; Plastoquinone-9:9, standard deviation; Replicate; Respiration; Treatment: pressure; Treatment: time after; Triacylglycerol, standard deviation; Triacylglycerols; Type of study; Ubiquinone-10:10; Ubiquinone-10:10, standard deviation |
| url | https://doi.org/10.1594/PANGAEA.960466 |