Microbial cell abundance in sinking diatom aggregates incubated in rotating pressure and control tanks
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| Auteurs principaux: | , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2023
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| _version_ | 1867169464294834176 |
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| author | Stief, Peter Middelboe, Mathias |
| author_facet | Stief, Peter Middelboe, Mathias |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level. The abundance of diatoms, bacteria, and viruses in sinking diatom aggregates was determined by microscopic (diatoms) and flow-cytometric cell counts (bacteria and viruses) in samples retrieved every 4 days throughout the 20-day incubation experiment. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_960471 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Microbial cell abundance in sinking diatom aggregates incubated in rotating pressure and control tanks Stief, Peter Middelboe, Mathias Bacteria; Bacteria, standard deviation; biological carbon pump; Date/time end, experiment; Date/time start, experiment; Deep sea; Diatom; Diatom abundance; Diatom abundance, standard deviation; Digital manometer, Keller AG, LEO5; Experiment; Experimental treatment; Flow-cytometric cell count, BD Biosciences, BD FACS Canto™ II; HADAL_aggregates; Hadal trench; hydrostatic pressure; Laboratory; Laboratory experiment; lipids; marine carbon cycle; marine snow; microbial community; Microscopic cell count, Fuchs-Rosenthal, Fuchs-Rosenthal counting chamber; Pigments; Replicate; Respiration; Treatment: pressure; Treatment: time after; Type of study; Viral abundance; Viral abundance, standard deviation The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level. The abundance of diatoms, bacteria, and viruses in sinking diatom aggregates was determined by microscopic (diatoms) and flow-cytometric cell counts (bacteria and viruses) in samples retrieved every 4 days throughout the 20-day incubation experiment. |
| title | Microbial cell abundance in sinking diatom aggregates incubated in rotating pressure and control tanks |
| topic | Bacteria; Bacteria, standard deviation; biological carbon pump; Date/time end, experiment; Date/time start, experiment; Deep sea; Diatom; Diatom abundance; Diatom abundance, standard deviation; Digital manometer, Keller AG, LEO5; Experiment; Experimental treatment; Flow-cytometric cell count, BD Biosciences, BD FACS Canto™ II; HADAL_aggregates; Hadal trench; hydrostatic pressure; Laboratory; Laboratory experiment; lipids; marine carbon cycle; marine snow; microbial community; Microscopic cell count, Fuchs-Rosenthal, Fuchs-Rosenthal counting chamber; Pigments; Replicate; Respiration; Treatment: pressure; Treatment: time after; Type of study; Viral abundance; Viral abundance, standard deviation |
| url | https://doi.org/10.1594/PANGAEA.960471 |