Exoenzyme activities associated with sinking diatom aggregates incubated in rotating pressure and control tanks
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| Main Authors: | , |
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2023
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| _version_ | 1867171327473876992 |
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| author | Balmonte, John Paul Franco-Cisterna, Belén |
| author_facet | Balmonte, John Paul Franco-Cisterna, Belén |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level. The activities of 5 exoenzymes (leucine-aminopeptidase, beta-glucosidase, laminarase, pullulanase, chondroitin sulfatase) associated with diatom aggregates were determined using fluorescently labeled substrates. Volumetric exoenzyme activities were calculated from the temporal change in fluorophore concentrations and the individual aggregate volume for samples retrieved every 4-8 days throughout the 20-day incubation experiment. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_960472 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Exoenzyme activities associated with sinking diatom aggregates incubated in rotating pressure and control tanks Balmonte, John Paul Franco-Cisterna, Belén beta-glucosidase activity; beta-glucosidase activity, standard deviation; biological carbon pump; Chondroitin sulfatase activity; Chondroitin sulfatase activity, standard deviation; Date/time end, experiment; Date/time start, experiment; Deep sea; Diatom; Digital manometer, Keller AG, LEO5; Experiment; Experimental treatment; Fluorimeter, Promega, Quantifluor mini fluorimeter; Gel permeation chromatography according to Arnosti 2003; HADAL_aggregates; Hadal trench; hydrostatic pressure; Laboratory; Laboratory experiment; Laminarase activity; Laminarase activity, standard deviation; Leucin-aminopeptidase activity; Leucin-aminopeptidase activity, standard deviation; lipids; marine carbon cycle; marine snow; microbial community; Pigments; Pullulanase activity; Pullulanase activity, standard deviation; Replicate; Respiration; Treatment: pressure; Treatment: time after; Type of study The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level. The activities of 5 exoenzymes (leucine-aminopeptidase, beta-glucosidase, laminarase, pullulanase, chondroitin sulfatase) associated with diatom aggregates were determined using fluorescently labeled substrates. Volumetric exoenzyme activities were calculated from the temporal change in fluorophore concentrations and the individual aggregate volume for samples retrieved every 4-8 days throughout the 20-day incubation experiment. |
| title | Exoenzyme activities associated with sinking diatom aggregates incubated in rotating pressure and control tanks |
| topic | beta-glucosidase activity; beta-glucosidase activity, standard deviation; biological carbon pump; Chondroitin sulfatase activity; Chondroitin sulfatase activity, standard deviation; Date/time end, experiment; Date/time start, experiment; Deep sea; Diatom; Digital manometer, Keller AG, LEO5; Experiment; Experimental treatment; Fluorimeter, Promega, Quantifluor mini fluorimeter; Gel permeation chromatography according to Arnosti 2003; HADAL_aggregates; Hadal trench; hydrostatic pressure; Laboratory; Laboratory experiment; Laminarase activity; Laminarase activity, standard deviation; Leucin-aminopeptidase activity; Leucin-aminopeptidase activity, standard deviation; lipids; marine carbon cycle; marine snow; microbial community; Pigments; Pullulanase activity; Pullulanase activity, standard deviation; Replicate; Respiration; Treatment: pressure; Treatment: time after; Type of study |
| url | https://doi.org/10.1594/PANGAEA.960472 |