Lipid content of liver and muscle tissue of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources

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Autores principales: Hörterer, Christina, Lannig, Gisela, Buck, Bela Hieronymus
Formato: Dataset Open Access
Lenguaje:en
Publicado: PANGAEA 2024
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author Hörterer, Christina
Lannig, Gisela
Buck, Bela Hieronymus
author_facet Hörterer, Christina
Lannig, Gisela
Buck, Bela Hieronymus
collection Datos científicos de ciencias marinas y ambientales
contents This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to July 2019 in the Centre for Aquaculture Research (ZAF) at the Alfred Wegener Institute for Polar and Marine research in Bremerhaven, Germany. The juvenile turbot (Scophthalmus maximus) were purchased from France Turbot (L'Épine, France) and acclimated to the recirculating aquaculture system (RAS) for 2 weeks prior to starting the 16 weeks experimental trial. To elucidate the effects of the protein sources and the level of FM replacement on the nutritional status of the fish lipid content of liver and muscle tissue were determined at the end of the experiment (t4). Muscle and liver samples (3 individual fish per tank; 15 fish per diet in total) were grinded under liquid nitrogen. Due to small volume, the individual samples of liver and muscle were pooled (n=5 tanks per diet) for the crude lipid content. Following the method of Folch et al. (1957) and Postel et al. (2000), the lipids in the muscle and liver tissue were extracted with 2:1 dichloromethanemethanol (v/v) and an aqueous solution of 0.88% potassium chloride (KCl) (w:v). Crude lipid content was determined gravimetrically to the nearest 0.001 g and calculated as the percentage of lipids of tissue wet weight.
format Dataset Open Access
id pangaea_https___doi_org_10_1594_PANGAEA_960775
institution PANGAEA
language en
publishDate 2024
publisher PANGAEA
record_format pangaea
spellingShingle Lipid content of liver and muscle tissue of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources
Hörterer, Christina
Lannig, Gisela
Buck, Bela Hieronymus
by-product; Experiment; glucose; glycogen; Identification; insect meal; Laboratory balance, Sartorius, Secura 5102-1S; Laboratory experiment; Lipid; Location; Method comment; Sample ID; Sampling date/time, experiment; Scophthalmus maximus, liver lipid, per dry mass; Scophthalmus maximus, muscle lipid, per dry mass; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Tank number; Time point, descriptive; Treatment; Type of study
This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to July 2019 in the Centre for Aquaculture Research (ZAF) at the Alfred Wegener Institute for Polar and Marine research in Bremerhaven, Germany. The juvenile turbot (Scophthalmus maximus) were purchased from France Turbot (L'Épine, France) and acclimated to the recirculating aquaculture system (RAS) for 2 weeks prior to starting the 16 weeks experimental trial. To elucidate the effects of the protein sources and the level of FM replacement on the nutritional status of the fish lipid content of liver and muscle tissue were determined at the end of the experiment (t4). Muscle and liver samples (3 individual fish per tank; 15 fish per diet in total) were grinded under liquid nitrogen. Due to small volume, the individual samples of liver and muscle were pooled (n=5 tanks per diet) for the crude lipid content. Following the method of Folch et al. (1957) and Postel et al. (2000), the lipids in the muscle and liver tissue were extracted with 2:1 dichloromethanemethanol (v/v) and an aqueous solution of 0.88% potassium chloride (KCl) (w:v). Crude lipid content was determined gravimetrically to the nearest 0.001 g and calculated as the percentage of lipids of tissue wet weight.
title Lipid content of liver and muscle tissue of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources
topic by-product; Experiment; glucose; glycogen; Identification; insect meal; Laboratory balance, Sartorius, Secura 5102-1S; Laboratory experiment; Lipid; Location; Method comment; Sample ID; Sampling date/time, experiment; Scophthalmus maximus, liver lipid, per dry mass; Scophthalmus maximus, muscle lipid, per dry mass; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Tank number; Time point, descriptive; Treatment; Type of study
url https://doi.org/10.1594/PANGAEA.960775