KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: biogenic silica concentrations from water samples
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2023
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| _version_ | 1867171039132254208 |
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| author | Thielecke, Antonia Smith, Alexandra Krause, Jeffrey W |
| author_facet | Thielecke, Antonia Smith, Alexandra Krause, Jeffrey W |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report biogenic Silica concentrations in the watercolumn collected during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). Samples (0.15-1L) were filtered onto polycarbonate filters (0.65 µm pore size, Whatman). The filters were then dried in the oven at 60 °C for 24 hours and measured following a modified procedure by DeMaster (1981, doi:10.1016/0016-7037(81)90006-5). A standard water bath (Fisher Scientific Isotemp Water Bath, Thermo Fisher Scientific, Waltham, US) was used to digest BSi samples at 85°C. The BSi analysis was carried out with a 4.5-hour time course alkaline digestion (0.1N Na2CO3) to dissolve the BSi followed by a 48 hour HF acid digestion (2.0M) to release the remaining lithogenic silica (LSi). Spectrophotometric analysis of the solubilized silica was done using a Thermo Scientific Genesys 10 UV- VIS Spectrophotometer (Thermo Fisher Scientific, Waltham, US). Model I linear regression was performed to calculate the concentrations of BSi while accounting for the amount of LSi that leached out during the alkaline digestion. Due to restrictions caused by the COVID-19 pandemic BSi data is only available for day 1 until day 17 and for day 31 until day 35. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_961209 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: biogenic silica concentrations from water samples Thielecke, Antonia Smith, Alexandra Krause, Jeffrey W AQUACOSM; biogenic silica; Biogenic silica; Biogenic silica, standard deviation; Coastal Upwelling System in a Changing Ocean; CUSCO; DATE/TIME; Day of experiment; Depth, water, experiment, bottom/maximum; Depth, water, experiment, top/minimum; Event label; Field experiment; Humboldt Current System; KOSMOS_2020; KOSMOS_2020_Mesocosm-M1; KOSMOS_2020_Mesocosm-M10; KOSMOS_2020_Mesocosm-M2; KOSMOS_2020_Mesocosm-M3; KOSMOS_2020_Mesocosm-M4; KOSMOS_2020_Mesocosm-M5; KOSMOS_2020_Mesocosm-M6; KOSMOS_2020_Mesocosm-M7; KOSMOS_2020_Mesocosm-M8; KOSMOS_2020_Mesocosm-M9; KOSMOS Peru; light limitation; Lithogenic silica; MESO; mesocosm experiment; Mesocosm experiment; Mesocosm label; Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean; Spectrophotometer UV/VIS, Thermo Fisher Scientific, Genesys 10; Treatment; Treatment: light condition; Type of study; Upwelling This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report biogenic Silica concentrations in the watercolumn collected during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). Samples (0.15-1L) were filtered onto polycarbonate filters (0.65 µm pore size, Whatman). The filters were then dried in the oven at 60 °C for 24 hours and measured following a modified procedure by DeMaster (1981, doi:10.1016/0016-7037(81)90006-5). A standard water bath (Fisher Scientific Isotemp Water Bath, Thermo Fisher Scientific, Waltham, US) was used to digest BSi samples at 85°C. The BSi analysis was carried out with a 4.5-hour time course alkaline digestion (0.1N Na2CO3) to dissolve the BSi followed by a 48 hour HF acid digestion (2.0M) to release the remaining lithogenic silica (LSi). Spectrophotometric analysis of the solubilized silica was done using a Thermo Scientific Genesys 10 UV- VIS Spectrophotometer (Thermo Fisher Scientific, Waltham, US). Model I linear regression was performed to calculate the concentrations of BSi while accounting for the amount of LSi that leached out during the alkaline digestion. Due to restrictions caused by the COVID-19 pandemic BSi data is only available for day 1 until day 17 and for day 31 until day 35. |
| title | KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: biogenic silica concentrations from water samples |
| topic | AQUACOSM; biogenic silica; Biogenic silica; Biogenic silica, standard deviation; Coastal Upwelling System in a Changing Ocean; CUSCO; DATE/TIME; Day of experiment; Depth, water, experiment, bottom/maximum; Depth, water, experiment, top/minimum; Event label; Field experiment; Humboldt Current System; KOSMOS_2020; KOSMOS_2020_Mesocosm-M1; KOSMOS_2020_Mesocosm-M10; KOSMOS_2020_Mesocosm-M2; KOSMOS_2020_Mesocosm-M3; KOSMOS_2020_Mesocosm-M4; KOSMOS_2020_Mesocosm-M5; KOSMOS_2020_Mesocosm-M6; KOSMOS_2020_Mesocosm-M7; KOSMOS_2020_Mesocosm-M8; KOSMOS_2020_Mesocosm-M9; KOSMOS Peru; light limitation; Lithogenic silica; MESO; mesocosm experiment; Mesocosm experiment; Mesocosm label; Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean; Spectrophotometer UV/VIS, Thermo Fisher Scientific, Genesys 10; Treatment; Treatment: light condition; Type of study; Upwelling |
| url | https://doi.org/10.1594/PANGAEA.961209 |