KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: HPLC pigment concentrations and phytoplankton community composition based on CHEMTAX analysis of water samples
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2023
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| _version_ | 1867169156483252224 |
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| author | Behncke, Jacqueline Thielecke, Antonia Kittu, Leila Georgieva, Silvia Nachtigall, Kerstin Hansen, Levka Fernández-Méndez, Mar |
| author_facet | Behncke, Jacqueline Thielecke, Antonia Kittu, Leila Georgieva, Silvia Nachtigall, Kerstin Hansen, Levka Fernández-Méndez, Mar |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report pigment concentrations and the phytoplankton community composition based on CHEMTAX analysis during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). To obtain pigment concentrations 0.15 – 1L of water sampled from the water column were filtered onto glass-fiber filters (precombusted at 450°C for 6h, GFF, 0.7 µm nominal pore size, Whatman) using a low vacuum of 200 mbar. Afterwards the filters were stored in cryovials at -80°C until analysis of photosynthetic pigments using reverse-phase high-performance liquid chromatography (HPLC) following (Barlow et al., 1997; doi:10.3354/meps161303) as described by (Paul et al., 2015; doi:10.5194/bg-19-5911-2022). The phytoplankton community composition was calculated with CHEMTAX, which classifies phytoplankton taxa based upon taxon-specific pigment ratios (Mackey et al., 1996; doi:10.3354/meps144265). The values for the Peruvian upwelling system determined by (DiTullio et al., 2005; doi:10.4319/LO.2005.50.6.1887) as described by (Meyer et al., 2017; doi:10.3389/fmars.2017.00001) were used as pigment ratios. As phytoplankton microscopy revealed high abundances of the raphydophite Fibrocapsa japonica, the initial pigment matrix was complemented by ratios for F. japonica from (Laza-Martinez et al., 2007; doi:10.1093/plankt/fbm069) from the Atlantic. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_961210 |
| institution | PANGAEA |
| language | en |
| publishDate | 2023 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: HPLC pigment concentrations and phytoplankton community composition based on CHEMTAX analysis of water samples Behncke, Jacqueline Thielecke, Antonia Kittu, Leila Georgieva, Silvia Nachtigall, Kerstin Hansen, Levka Fernández-Méndez, Mar 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; AQUACOSM; Chemtax; Chlorophyll a; Chlorophyll a, Chlorophyta; Chlorophyll a, Coccolithophores; Chlorophyll a, Cryptophyta; Chlorophyll a, Diatoms; Chlorophyll a, Dinoflagellata; Chlorophyll a, Fibrocapsa japonica; Chlorophyll a, Pelagophytes; Chlorophyll a, Phaeocystis; Chlorophyll a, Prasinophyta; Chlorophyll a, Synechococcus spp.; Chlorophyll b; Chlorophyll c3; Coastal Upwelling System in a Changing Ocean; CUSCO; DATE/TIME; Day of experiment; Depth, water, experiment, bottom/maximum; Depth, water, experiment, top/minimum; Event label; Field experiment; Fucoxanthin; HPLC/CHEMTAX (Mackey et al. 1996); HPLC pigments; KOSMOS_2020; KOSMOS_2020_Mesocosm-M1; KOSMOS_2020_Mesocosm-M10; KOSMOS_2020_Mesocosm-M2; KOSMOS_2020_Mesocosm-M3; KOSMOS_2020_Mesocosm-M4; KOSMOS_2020_Mesocosm-M5; KOSMOS_2020_Mesocosm-M6; KOSMOS_2020_Mesocosm-M7; KOSMOS_2020_Mesocosm-M8; KOSMOS_2020_Mesocosm-M9; KOSMOS Peru; light limitation; Lutein; MESO; mesocosm experiment; Mesocosm experiment; Mesocosm label; Neoxanthin; Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean; Peridinin; Phytoplankton composition; Prasinoxanthin; Treatment; Treatment: light condition; Type of study; Upwelling; Violaxanthin; Zeaxanthin This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report pigment concentrations and the phytoplankton community composition based on CHEMTAX analysis during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). To obtain pigment concentrations 0.15 – 1L of water sampled from the water column were filtered onto glass-fiber filters (precombusted at 450°C for 6h, GFF, 0.7 µm nominal pore size, Whatman) using a low vacuum of 200 mbar. Afterwards the filters were stored in cryovials at -80°C until analysis of photosynthetic pigments using reverse-phase high-performance liquid chromatography (HPLC) following (Barlow et al., 1997; doi:10.3354/meps161303) as described by (Paul et al., 2015; doi:10.5194/bg-19-5911-2022). The phytoplankton community composition was calculated with CHEMTAX, which classifies phytoplankton taxa based upon taxon-specific pigment ratios (Mackey et al., 1996; doi:10.3354/meps144265). The values for the Peruvian upwelling system determined by (DiTullio et al., 2005; doi:10.4319/LO.2005.50.6.1887) as described by (Meyer et al., 2017; doi:10.3389/fmars.2017.00001) were used as pigment ratios. As phytoplankton microscopy revealed high abundances of the raphydophite Fibrocapsa japonica, the initial pigment matrix was complemented by ratios for F. japonica from (Laza-Martinez et al., 2007; doi:10.1093/plankt/fbm069) from the Atlantic. |
| title | KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: HPLC pigment concentrations and phytoplankton community composition based on CHEMTAX analysis of water samples |
| topic | 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; AQUACOSM; Chemtax; Chlorophyll a; Chlorophyll a, Chlorophyta; Chlorophyll a, Coccolithophores; Chlorophyll a, Cryptophyta; Chlorophyll a, Diatoms; Chlorophyll a, Dinoflagellata; Chlorophyll a, Fibrocapsa japonica; Chlorophyll a, Pelagophytes; Chlorophyll a, Phaeocystis; Chlorophyll a, Prasinophyta; Chlorophyll a, Synechococcus spp.; Chlorophyll b; Chlorophyll c3; Coastal Upwelling System in a Changing Ocean; CUSCO; DATE/TIME; Day of experiment; Depth, water, experiment, bottom/maximum; Depth, water, experiment, top/minimum; Event label; Field experiment; Fucoxanthin; HPLC/CHEMTAX (Mackey et al. 1996); HPLC pigments; KOSMOS_2020; KOSMOS_2020_Mesocosm-M1; KOSMOS_2020_Mesocosm-M10; KOSMOS_2020_Mesocosm-M2; KOSMOS_2020_Mesocosm-M3; KOSMOS_2020_Mesocosm-M4; KOSMOS_2020_Mesocosm-M5; KOSMOS_2020_Mesocosm-M6; KOSMOS_2020_Mesocosm-M7; KOSMOS_2020_Mesocosm-M8; KOSMOS_2020_Mesocosm-M9; KOSMOS Peru; light limitation; Lutein; MESO; mesocosm experiment; Mesocosm experiment; Mesocosm label; Neoxanthin; Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean; Peridinin; Phytoplankton composition; Prasinoxanthin; Treatment; Treatment: light condition; Type of study; Upwelling; Violaxanthin; Zeaxanthin |
| url | https://doi.org/10.1594/PANGAEA.961210 |