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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2024
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| Online Access: | https://doi.org/10.1594/PANGAEA.964004 |
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| _version_ | 1867171329913913344 |
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| author | Dursun, Fuat Krock, Bernd |
| author_facet | Dursun, Fuat Krock, Bernd |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Phycotoxins (Fuat Dursun, Bernd Krock): An 8 L aliquot of pooled water from surface water, 10 m depth and the thermocline was filtered through 5 μm polycarbonate filters for azaspiracid and karlotoxin determination. The filters were extracted by repeated rinsing with methanol, the methanolic extracts were transferred to centrifugation filters and filtered at a cutt-off of 0.45 μm by centrifugation. Filtrates were transferred into analytical glass vials for posterior LC-MS/MS determination at AWI. Net tows of each station were combined and ajusted to 1 L with filtrated seawater. Aliquots of 200 mL, 50 mL, and 20 mL were taken for DNA analysis, Cell isolation, and fixation, respectively. The remaining aliquot of the net tow concentrate was filtered through a filter array of 200, 50, and 20 μm meshes. The residue of each mesh was rinsed with filtrated seawater into a centrifugation tube, homogenized by shaking, and divided into two aliquots. Both aliquots of each size fraction were centrifuged at maximum speed for 15 min. After centrifugation, supernatants were decanted and the remaining cell pellets resuspended with a small volume of filtrated seawater and transferred to cryovials containing ceramic beads. The samples were centrifuged again and supernatants carefully removed with a pipette. To one of the two size fraction aliquots 500 μL methanol was added and the same volume of dilute acetic acid to the other. All samples were homogenized with FastPrep instrument by reciprocal shaking at maximum speed and homogenated were centrifuged. After centrifugation, the toxin containing extracts were removed and transferred to centrifugation filters. The extracts were filtered by centrifugation and the filtered extracts were transferred into analytical glass vials for LC-MS/MS and LC-FLD determination, respectively, in the home lab. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_964004 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Phycotoxins in water sampled during the PHYCOB cruise in the Southwestern Black Sea in 2021 Dursun, Fuat Krock, Bernd chlorophyll-a; CTD profile; Depth, bottom/max; Depth, top/min; Event label; Goniodomin A per net tow; Gonyautoxins 2/3 per net tow; HAB species; isolated strains; Latitude of event; Liquid Chromatography with tandem mass spectrometry (LC-MS/MS); Longitude of event; MULT; Multiple investigations; net tows; NOC; Nutrient data; Pectenotoxin 2 per net tow; Pectenotoxin 2sa per net tow; PHYCOB; PHYCOB_1; PHYCOB_10; PHYCOB_11; PHYCOB_12; PHYCOB_13; PHYCOB_14; PHYCOB_15; PHYCOB_16; PHYCOB_17; PHYCOB_18; PHYCOB_19; PHYCOB_2; PHYCOB_20; PHYCOB_21; PHYCOB_22; PHYCOB_23; PHYCOB_3; PHYCOB_4; PHYCOB_5; PHYCOB_6; PHYCOB_7; PHYCOB_8; PHYCOB_9; phycotoxins; POC; Sample ID; Tübitak Marmara; vitamin B12; Yessotoxin 1061 per net tow; Yessotoxin 1131 per net tow; Yessotoxin 1155 per net tow; Yessotoxin 1173 per net tow; Yessotoxin 1273 per net tow; Yessotoxin 1405 per net tow; Yessotoxin per net tow Phycotoxins (Fuat Dursun, Bernd Krock): An 8 L aliquot of pooled water from surface water, 10 m depth and the thermocline was filtered through 5 μm polycarbonate filters for azaspiracid and karlotoxin determination. The filters were extracted by repeated rinsing with methanol, the methanolic extracts were transferred to centrifugation filters and filtered at a cutt-off of 0.45 μm by centrifugation. Filtrates were transferred into analytical glass vials for posterior LC-MS/MS determination at AWI. Net tows of each station were combined and ajusted to 1 L with filtrated seawater. Aliquots of 200 mL, 50 mL, and 20 mL were taken for DNA analysis, Cell isolation, and fixation, respectively. The remaining aliquot of the net tow concentrate was filtered through a filter array of 200, 50, and 20 μm meshes. The residue of each mesh was rinsed with filtrated seawater into a centrifugation tube, homogenized by shaking, and divided into two aliquots. Both aliquots of each size fraction were centrifuged at maximum speed for 15 min. After centrifugation, supernatants were decanted and the remaining cell pellets resuspended with a small volume of filtrated seawater and transferred to cryovials containing ceramic beads. The samples were centrifuged again and supernatants carefully removed with a pipette. To one of the two size fraction aliquots 500 μL methanol was added and the same volume of dilute acetic acid to the other. All samples were homogenized with FastPrep instrument by reciprocal shaking at maximum speed and homogenated were centrifuged. After centrifugation, the toxin containing extracts were removed and transferred to centrifugation filters. The extracts were filtered by centrifugation and the filtered extracts were transferred into analytical glass vials for LC-MS/MS and LC-FLD determination, respectively, in the home lab. |
| title | Phycotoxins in water sampled during the PHYCOB cruise in the Southwestern Black Sea in 2021 |
| topic | chlorophyll-a; CTD profile; Depth, bottom/max; Depth, top/min; Event label; Goniodomin A per net tow; Gonyautoxins 2/3 per net tow; HAB species; isolated strains; Latitude of event; Liquid Chromatography with tandem mass spectrometry (LC-MS/MS); Longitude of event; MULT; Multiple investigations; net tows; NOC; Nutrient data; Pectenotoxin 2 per net tow; Pectenotoxin 2sa per net tow; PHYCOB; PHYCOB_1; PHYCOB_10; PHYCOB_11; PHYCOB_12; PHYCOB_13; PHYCOB_14; PHYCOB_15; PHYCOB_16; PHYCOB_17; PHYCOB_18; PHYCOB_19; PHYCOB_2; PHYCOB_20; PHYCOB_21; PHYCOB_22; PHYCOB_23; PHYCOB_3; PHYCOB_4; PHYCOB_5; PHYCOB_6; PHYCOB_7; PHYCOB_8; PHYCOB_9; phycotoxins; POC; Sample ID; Tübitak Marmara; vitamin B12; Yessotoxin 1061 per net tow; Yessotoxin 1131 per net tow; Yessotoxin 1155 per net tow; Yessotoxin 1173 per net tow; Yessotoxin 1273 per net tow; Yessotoxin 1405 per net tow; Yessotoxin per net tow |
| url | https://doi.org/10.1594/PANGAEA.964004 |