KOSMOS 2018 Gran Canaria mesocosm study: prokaryotes
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| Natura: | Dataset Open Access |
| Lingua: | en |
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PANGAEA
2024
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| _version_ | 1867171041212628992 |
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| author | Gómez-Letona, Markel Sebastian, Marta Montero, María Fernanda Riebesell, Ulf Arístegui, Javier |
| author_facet | Gómez-Letona, Markel Sebastian, Marta Montero, María Fernanda Riebesell, Ulf Arístegui, Javier |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This dataset contains the results of the analysis of standing stocks and activity of prokaryotes during the KOSMOS mesocosm experiment carried out in the framework of the Ocean Artificial Upwelling project. The experiment was carried out in the autumn of 2018 in the oligotrophic waters off Gran Canaria. During the 39 days of experiment nutrient-rich deep water was added to the mesocosms in two modes (singular vs recurring additions), with four levels of intensity. Prokaryotic abundances and viability were quantified with a FACSCalibur flow cytometer (Becton-Dickinson). Prokaryotic activity (as protein synthesis) was assessed by means of BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT), using a Zeiss Axio Imager.Z2m Epifluorescence Microscope (AxioCam MRm, Carl Zeiss MicroImaging). The aim of this dataset was to study the effect of different upwelling regimes on the prokaryotic communities and its potential implications for organic matter cycling. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_964544 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | KOSMOS 2018 Gran Canaria mesocosm study: prokaryotes Gómez-Letona, Markel Sebastian, Marta Montero, María Fernanda Riebesell, Ulf Arístegui, Javier Abundance; activity; artificial upwelling; Canarias Sea; DATE/TIME; Day of experiment; Depth, water, experiment, bottom/maximum; Depth, water, experiment, top/minimum; Epifluorescence microscope, Carl Zeiss MicroImaging, AxioCam MRm, Zeiss Axio Imager.Z2m; Event label; FACSCalibur flow-cytometer (Becton Dickinson); Field experiment; High nucleic acid prokaryotes; High nucleic acid prokaryotes/prokaryotes ratio; KOSMOS_2018; KOSMOS_2018_Mesocosm-A; KOSMOS_2018_Mesocosm-DW; KOSMOS_2018_Mesocosm-M1; KOSMOS_2018_Mesocosm-M2; KOSMOS_2018_Mesocosm-M3; KOSMOS_2018_Mesocosm-M4; KOSMOS_2018_Mesocosm-M5; KOSMOS_2018_Mesocosm-M6; KOSMOS_2018_Mesocosm-M7; KOSMOS_2018_Mesocosm-M8; KOSMOS_2018_Mesocosm-M9; KOSMOS Gran Canaria; Low nucleic acid prokaryotes; MESO; mesocosm experiment; Mesocosm experiment; Mesocosm label; Nanoeukaryotes; Ocean Artificial Upwelling; Ocean-artUp; Picoeukaryotes; Prochlorococcus; prokaryote; Prokaryotes; Prokaryotes, active; Prokaryotes, active, standard deviation; protein; Synechococcus; Treatment; Treatment: nitrogen; Type of study; viability; Viability This dataset contains the results of the analysis of standing stocks and activity of prokaryotes during the KOSMOS mesocosm experiment carried out in the framework of the Ocean Artificial Upwelling project. The experiment was carried out in the autumn of 2018 in the oligotrophic waters off Gran Canaria. During the 39 days of experiment nutrient-rich deep water was added to the mesocosms in two modes (singular vs recurring additions), with four levels of intensity. Prokaryotic abundances and viability were quantified with a FACSCalibur flow cytometer (Becton-Dickinson). Prokaryotic activity (as protein synthesis) was assessed by means of BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT), using a Zeiss Axio Imager.Z2m Epifluorescence Microscope (AxioCam MRm, Carl Zeiss MicroImaging). The aim of this dataset was to study the effect of different upwelling regimes on the prokaryotic communities and its potential implications for organic matter cycling. |
| title | KOSMOS 2018 Gran Canaria mesocosm study: prokaryotes |
| topic | Abundance; activity; artificial upwelling; Canarias Sea; DATE/TIME; Day of experiment; Depth, water, experiment, bottom/maximum; Depth, water, experiment, top/minimum; Epifluorescence microscope, Carl Zeiss MicroImaging, AxioCam MRm, Zeiss Axio Imager.Z2m; Event label; FACSCalibur flow-cytometer (Becton Dickinson); Field experiment; High nucleic acid prokaryotes; High nucleic acid prokaryotes/prokaryotes ratio; KOSMOS_2018; KOSMOS_2018_Mesocosm-A; KOSMOS_2018_Mesocosm-DW; KOSMOS_2018_Mesocosm-M1; KOSMOS_2018_Mesocosm-M2; KOSMOS_2018_Mesocosm-M3; KOSMOS_2018_Mesocosm-M4; KOSMOS_2018_Mesocosm-M5; KOSMOS_2018_Mesocosm-M6; KOSMOS_2018_Mesocosm-M7; KOSMOS_2018_Mesocosm-M8; KOSMOS_2018_Mesocosm-M9; KOSMOS Gran Canaria; Low nucleic acid prokaryotes; MESO; mesocosm experiment; Mesocosm experiment; Mesocosm label; Nanoeukaryotes; Ocean Artificial Upwelling; Ocean-artUp; Picoeukaryotes; Prochlorococcus; prokaryote; Prokaryotes; Prokaryotes, active; Prokaryotes, active, standard deviation; protein; Synechococcus; Treatment; Treatment: nitrogen; Type of study; viability; Viability |
| url | https://doi.org/10.1594/PANGAEA.964544 |