Gene expression of protease genes in larval Strongylocentrotus purpuratus during laboratory experiments with different food treatments
Fuente:
PANGAEA
Salvato in:
| Autori principali: | , , , |
|---|---|
| Natura: | Dataset Open Access |
| Lingua: | en |
| Pubblicazione: |
PANGAEA
2024
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1867169469740089344 |
|---|---|
| author | Hildebrand, Jasper Chang, William Weijen Hu, Marian Y Stumpp, Meike |
| author_facet | Hildebrand, Jasper Chang, William Weijen Hu, Marian Y Stumpp, Meike |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_965918 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Gene expression of protease genes in larval Strongylocentrotus purpuratus during laboratory experiments with different food treatments Hildebrand, Jasper Chang, William Weijen Hu, Marian Y Stumpp, Meike algae; culture experiment; Date/time start, experiment; DIVER; energetics; Experiment; La_Jolla_S_purpuratus; Laboratory; Laboratory experiment; La Jolla, USA; Microscope, Zeiss, Axio Imager Z; measured with Hemocytometer; physiology; protein; Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1; Sample ID; Sampling by diver; sea urchin; Strongylocentrotus purpuratus; Strongylocentrotus purpuratus, larvae, aminopeptidase N, relative gene expression; Strongylocentrotus purpuratus, larvae, carboxypeptidase B, relative gene expression; Strongylocentrotus purpuratus, larvae, enteropeptidase, relative gene expression; Strongylocentrotus purpuratus, larvae, trypsin II, relative gene expression; Strongylocentrotus purpuratus, larval age; Treatment: food; Type of study Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology. |
| title | Gene expression of protease genes in larval Strongylocentrotus purpuratus during laboratory experiments with different food treatments |
| topic | algae; culture experiment; Date/time start, experiment; DIVER; energetics; Experiment; La_Jolla_S_purpuratus; Laboratory; Laboratory experiment; La Jolla, USA; Microscope, Zeiss, Axio Imager Z; measured with Hemocytometer; physiology; protein; Real-time-PCR-system, ThermoFisher Scientific, QuantStudio 1; Sample ID; Sampling by diver; sea urchin; Strongylocentrotus purpuratus; Strongylocentrotus purpuratus, larvae, aminopeptidase N, relative gene expression; Strongylocentrotus purpuratus, larvae, carboxypeptidase B, relative gene expression; Strongylocentrotus purpuratus, larvae, enteropeptidase, relative gene expression; Strongylocentrotus purpuratus, larvae, trypsin II, relative gene expression; Strongylocentrotus purpuratus, larval age; Treatment: food; Type of study |
| url | https://doi.org/10.1594/PANGAEA.965918 |