Quantification of microbial cells and virus-like particles in the water column above Atacama Trench
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| Auteurs principaux: | , , |
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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2024
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| _version_ | 1867171889276780544 |
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| author | Schauberger, Clemens Middelboe, Mathias Glud, Ronnie N |
| author_facet | Schauberger, Clemens Middelboe, Mathias Glud, Ronnie N |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_965921 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Quantification of microbial cells and virus-like particles in the water column above Atacama Trench Schauberger, Clemens Middelboe, Mathias Glud, Ronnie N Abundance; Bacteria; CTD/Rosette; CTD-RO; Date/Time of event; Deep sea; DEPTH, water; Elevation of event; Event label; Flow cytometry system, Becton Dickinson, FACSCanto II; Hadal trench; Latitude of event; Longitude of event; Microbial abundance, cells; Microbial abundance, standard deviation; prokaryote; SO261; SO261_108-1; SO261_110-1; SO261_19-1; SO261_34-1; SO261_47-1; SO261_62-1; SO261_7-2; SO261_74-1; SO261_80-1; SO261_83-1; Sonne_2; Station label; Viral abundance; Viral abundance, standard deviation; virus-like particles; Water sample; WS Water samples were collected using 7.5-Liter Niskin bottles and fixed with 25% electron microscopy graded glutaraldehyde (1% final concentration) and stored at -80°C until quantification by flow cytometry. Samples were measured in triplicates using a BD FACSCanto™ II flow cytometer, after staining with SYBR Green I (Brussaard 2004). The flow rate was 5–7 μl/min, as determined by BD Trucount™ Beads. The laser settings and gating examples can be found in the Supporting Information of Schauberger et al. (2021). |
| title | Quantification of microbial cells and virus-like particles in the water column above Atacama Trench |
| topic | Abundance; Bacteria; CTD/Rosette; CTD-RO; Date/Time of event; Deep sea; DEPTH, water; Elevation of event; Event label; Flow cytometry system, Becton Dickinson, FACSCanto II; Hadal trench; Latitude of event; Longitude of event; Microbial abundance, cells; Microbial abundance, standard deviation; prokaryote; SO261; SO261_108-1; SO261_110-1; SO261_19-1; SO261_34-1; SO261_47-1; SO261_62-1; SO261_7-2; SO261_74-1; SO261_80-1; SO261_83-1; Sonne_2; Station label; Viral abundance; Viral abundance, standard deviation; virus-like particles; Water sample; WS |
| url | https://doi.org/10.1594/PANGAEA.965921 |