Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea
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| Autores principales: | , |
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2024
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| _version_ | 1867168524470845440 |
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| author | Leys, Sally P Federwisch, Luisa |
| author_facet | Leys, Sally P Federwisch, Luisa |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_966335 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea Leys, Sally P Federwisch, Luisa Agassiz Trawl; AGT; Antarctic; AWI_BPP; Bentho-Pelagic Processes @ AWI; Demospongiae; Elevation of event; Event label; Hexactinellida; Image; Image number/name; James Clark Ross; JR17003a; JR17003A; JR17003A_38; JR17003A_46; JR17003A_52; Latitude of event; Longitude of event; microbes; microbiome; microscopy; Prince Gustav Channel; Sample ID; Sponges; TEM; Transmission electron microscope (TEM); transmission electron microscopy; ultrastructure; Weddell Sea This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale. |
| title | Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
| topic | Agassiz Trawl; AGT; Antarctic; AWI_BPP; Bentho-Pelagic Processes @ AWI; Demospongiae; Elevation of event; Event label; Hexactinellida; Image; Image number/name; James Clark Ross; JR17003a; JR17003A; JR17003A_38; JR17003A_46; JR17003A_52; Latitude of event; Longitude of event; microbes; microbiome; microscopy; Prince Gustav Channel; Sample ID; Sponges; TEM; Transmission electron microscope (TEM); transmission electron microscopy; ultrastructure; Weddell Sea |
| url | https://doi.org/10.1594/PANGAEA.966335 |