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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2024
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| Subjects: | |
| Online Access: | https://doi.org/10.1594/PANGAEA.966611 |
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| _version_ | 1867170123860672512 |
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| author | Gutiérrez-Zárate, Cristina Veiga, Alfredo Gori, Andrea Gerpe, Fabián Domínguez, Leo Rodríguez, Alberto Arias, Noelia Álvarez, Marta Acerbi, Rubén Vázquez, Lucía Movilla, Juancho Varela, Marta M Alba-Salgueiro, Rodrigo Valencia-Vila, Joaquín Orejas, Covadonga |
| author_facet | Gutiérrez-Zárate, Cristina Veiga, Alfredo Gori, Andrea Gerpe, Fabián Domínguez, Leo Rodríguez, Alberto Arias, Noelia Álvarez, Marta Acerbi, Rubén Vázquez, Lucía Movilla, Juancho Varela, Marta M Alba-Salgueiro, Rodrigo Valencia-Vila, Joaquín Orejas, Covadonga |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Prokaryotes were quantified by flow citometry (CytoFLEXflex S, Beckman Coulter) as previously described by Gasol et al. (1999) with our own adjustment of the scatter settings to more accurately resolve these cells with the new CytoFLEX flow cytometry technology. Measurements were performed every month from different parts of the aquaria setup: the input seawater, the reservoir tank (after the filtration of seawater to 5 µm and before the UV lamp) and every experimental aquaria. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_966611 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Prokariotic abundances during a multi-stressor aquaria experiment with the cold-water coral Dendrophyllia cornigera Gutiérrez-Zárate, Cristina Veiga, Alfredo Gori, Andrea Gerpe, Fabián Domínguez, Leo Rodríguez, Alberto Arias, Noelia Álvarez, Marta Acerbi, Rubén Vázquez, Lucía Movilla, Juancho Varela, Marta M Alba-Salgueiro, Rodrigo Valencia-Vila, Joaquín Orejas, Covadonga Accidental bycatch sampling; Atlantic Ocean; Comment; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; Flow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R); flow cytometry; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Prokaryotes; prokaryotic abundance; Replicate; Species; Species, unique identification (Semantic URI); Species, unique identification (URI); Type of study A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Prokaryotes were quantified by flow citometry (CytoFLEXflex S, Beckman Coulter) as previously described by Gasol et al. (1999) with our own adjustment of the scatter settings to more accurately resolve these cells with the new CytoFLEX flow cytometry technology. Measurements were performed every month from different parts of the aquaria setup: the input seawater, the reservoir tank (after the filtration of seawater to 5 µm and before the UV lamp) and every experimental aquaria. |
| title | Prokariotic abundances during a multi-stressor aquaria experiment with the cold-water coral Dendrophyllia cornigera |
| topic | Accidental bycatch sampling; Atlantic Ocean; Comment; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; Flow cytometer, Beckman Coulter, CytoFLEX S (N-V-B-R); flow cytometry; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Prokaryotes; prokaryotic abundance; Replicate; Species; Species, unique identification (Semantic URI); Species, unique identification (URI); Type of study |
| url | https://doi.org/10.1594/PANGAEA.966611 |