Cellular stress biomarkers result of the cold-water coral Viminella flagellum during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification
Fuente:
PANGAEA
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Dataset Open Access |
| Lenguaje: | en |
| Publicado: |
PANGAEA
2024
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1867169159578648576 |
|---|---|
| author | Martins, Ines Mano, Beatriz Godinho, António Goulart, Joana Sire de Vilar, Anaïs Carreiro-Silva, Marina |
| author_facet | Martins, Ines Mano, Beatriz Godinho, António Goulart, Joana Sire de Vilar, Anaïs Carreiro-Silva, Marina |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We report the results of a land-based experiment testing the cumulative effects of copper (Cu) exposure and ocean acidification (OA). Corals were obtained as by-catch during an experimental long-line fisheries campaign (CONDOR monitoring program, Okeanos-University of the Azores) on the Condor Seamount (38°54′N, 29°05′W), Azores Archipelago, at 200 m depth in October 2019. The experiment was undertaken at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores) where corals were exposed to four OA/Cu-contamination scenarios: (1) ambient pCO2/pH level as measured in situ conditions (385 μatm/ pH 8.09); (2) high pCO2/reduced pH (IPCC RCP8.5 scenario, 1000 μatm/ pH 7.73); (3) ambient pCO2/pH level and additional Cu concentration (60 µg/l); (4) high pCO2/reduced pH and additional Cu concentration (60 µg/l). The pH/pCO2 modification was achieved by bubbling seawater with either pure CO2 (to increase pCO2) or CO2 low air (to decrease pCO2). The copper concentration used in this experiment followed a trial simulating a polymetallic particles plume release during a potential deep-sea mining event. This sublethal concentration was found to be the highest copper concentration dissolved in seawater. Metabolic (CA and Hsp70), oxidative damage (SOD, MDA) and detoxification (GST) biomarkers were measured in 4 corals from each treatment at the end of the experiment, using a multiplate spectrophotometer reader. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_966771 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Cellular stress biomarkers result of the cold-water coral Viminella flagellum during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification Martins, Ines Mano, Beatriz Godinho, António Goulart, Joana Sire de Vilar, Anaïs Carreiro-Silva, Marina Aquaria ID; Azores; Azores_Deep_Octocoral_Sampling_Viminella_flagellum; Carbonic anhydrase activity, unit per protein mass; Colony number/ID; Condor Seamounts; Experiment; Glutathione S-transferase, activity, per protein mass; Heat shock protein 70, per protein mass; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; Malondialdehyde, per protein mass; Multiskan Microplate spectrophotometer, Thermo Scientific™, GO; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Superoxide dismutase activity, unit per protein mass; Time in days; Treatment; Viminella_flagellum_Aquaria_Experiment We report the results of a land-based experiment testing the cumulative effects of copper (Cu) exposure and ocean acidification (OA). Corals were obtained as by-catch during an experimental long-line fisheries campaign (CONDOR monitoring program, Okeanos-University of the Azores) on the Condor Seamount (38°54′N, 29°05′W), Azores Archipelago, at 200 m depth in October 2019. The experiment was undertaken at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores) where corals were exposed to four OA/Cu-contamination scenarios: (1) ambient pCO2/pH level as measured in situ conditions (385 μatm/ pH 8.09); (2) high pCO2/reduced pH (IPCC RCP8.5 scenario, 1000 μatm/ pH 7.73); (3) ambient pCO2/pH level and additional Cu concentration (60 µg/l); (4) high pCO2/reduced pH and additional Cu concentration (60 µg/l). The pH/pCO2 modification was achieved by bubbling seawater with either pure CO2 (to increase pCO2) or CO2 low air (to decrease pCO2). The copper concentration used in this experiment followed a trial simulating a polymetallic particles plume release during a potential deep-sea mining event. This sublethal concentration was found to be the highest copper concentration dissolved in seawater. Metabolic (CA and Hsp70), oxidative damage (SOD, MDA) and detoxification (GST) biomarkers were measured in 4 corals from each treatment at the end of the experiment, using a multiplate spectrophotometer reader. |
| title | Cellular stress biomarkers result of the cold-water coral Viminella flagellum during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification |
| topic | Aquaria ID; Azores; Azores_Deep_Octocoral_Sampling_Viminella_flagellum; Carbonic anhydrase activity, unit per protein mass; Colony number/ID; Condor Seamounts; Experiment; Glutathione S-transferase, activity, per protein mass; Heat shock protein 70, per protein mass; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; Malondialdehyde, per protein mass; Multiskan Microplate spectrophotometer, Thermo Scientific™, GO; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Superoxide dismutase activity, unit per protein mass; Time in days; Treatment; Viminella_flagellum_Aquaria_Experiment |
| url | https://doi.org/10.1594/PANGAEA.966771 |