Seawater carbonate chemistry and oxidative stress response, carbon metabolism and rapid light curve fitted parameters of phytoplankton Phaeodactylum tricornutum
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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2024
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| _version_ | 1867169159597522944 |
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| author | Moreno, Hugo Rokitta, Sebastian D Tremblay, Nelly Boersma, Maarten Groß, Elisabeth Klip, Helena Wiltshire, Karen Helen Meunier, Cédric Léo |
| author_facet | Moreno, Hugo Rokitta, Sebastian D Tremblay, Nelly Boersma, Maarten Groß, Elisabeth Klip, Helena Wiltshire, Karen Helen Meunier, Cédric Léo |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also quantified the concentration of photosynthetic pigments chlorophyll a (Chl-a) and fucoxanthin. We used least-squares fitting on the obtained data to derive physiological photosynthesis parameters, such as compensation point, as well as photochemical efficiency (α), light acclimation index (Ik) and maximum net photosynthesis rate (Vmax). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_966813 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and oxidative stress response, carbon metabolism and rapid light curve fitted parameters of phytoplankton Phaeodactylum tricornutum Moreno, Hugo Rokitta, Sebastian D Tremblay, Nelly Boersma, Maarten Groß, Elisabeth Klip, Helena Wiltshire, Karen Helen Meunier, Cédric Léo Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacillariophyta; beta-Carotene per cell; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, dissolved exudation, per cell; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon per cell; Catalase activity, unit per protein mass; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Compensation point; Compensation point, standard deviation; Diadinoxanthin per cell; Fucoxanthin per cell; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione peroxidase activity, unit per protein mass; Glutathione S-transferase activity, unit per protein mass; Growth/Morphology; Growth rate; Initial slope of rapid light curve; Initial slope of rapid light curve, standard deviation; Laboratory experiment; Laboratory strains; Light saturation point; Light saturation point, standard deviation; Macro-nutrients; Malondialdehyde, per wet mass; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Nitrogen/Phosphorus ratio; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Phaeodactylum tricornutum; Phosphorus, organic, particulate, production per cell; Phytoplankton; Primary production/Photosynthesis; Replicate; Respiration; Respiration rate, carbon; Salinity; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Superoxide dismutase manganese activity, unit per protein mass; Temperature; Temperature, water; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Violaxanthin per cell We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also quantified the concentration of photosynthetic pigments chlorophyll a (Chl-a) and fucoxanthin. We used least-squares fitting on the obtained data to derive physiological photosynthesis parameters, such as compensation point, as well as photochemical efficiency (α), light acclimation index (Ik) and maximum net photosynthesis rate (Vmax). |
| title | Seawater carbonate chemistry and oxidative stress response, carbon metabolism and rapid light curve fitted parameters of phytoplankton Phaeodactylum tricornutum |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacillariophyta; beta-Carotene per cell; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, dissolved exudation, per cell; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon per cell; Catalase activity, unit per protein mass; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chromista; Compensation point; Compensation point, standard deviation; Diadinoxanthin per cell; Fucoxanthin per cell; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Glutathione peroxidase activity, unit per protein mass; Glutathione S-transferase activity, unit per protein mass; Growth/Morphology; Growth rate; Initial slope of rapid light curve; Initial slope of rapid light curve, standard deviation; Laboratory experiment; Laboratory strains; Light saturation point; Light saturation point, standard deviation; Macro-nutrients; Malondialdehyde, per wet mass; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Nitrogen/Phosphorus ratio; Not applicable; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH, standard deviation; pH, total scale; Phaeodactylum tricornutum; Phosphorus, organic, particulate, production per cell; Phytoplankton; Primary production/Photosynthesis; Replicate; Respiration; Respiration rate, carbon; Salinity; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Strain; Superoxide dismutase manganese activity, unit per protein mass; Temperature; Temperature, water; Treatment: partial pressure of carbon dioxide; Treatment: temperature; Type of study; Violaxanthin per cell |
| url | https://doi.org/10.1594/PANGAEA.966813 |