Seawater carbonate chemistry and gene function and transposon activity of juvenile subarctic crustacean
Fuente:
PANGAEA
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Dataset Open Access |
| Sprache: | en |
| Veröffentlicht: |
PANGAEA
2024
|
| Schlagworte: | |
| Online-Zugang: | |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1867169160107130880 |
|---|---|
| author | Spencer, Laura H Long, William Christopher Spies, Ingrid B Nichols, Krista M Foy, Robert J |
| author_facet | Spencer, Laura H Long, William Christopher Spies, Ingrid B Nichols, Krista M Foy, Robert J |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | In this study, we used functional genomics to examine the molecular response of OA exposed red king crab. We leveraged juveniles that were exposed to (and tolerated) three carbonate chemistry treatments from hatching to the first crab stage (C1), thus capturing transcriptional differences among crab that are reared in historically ambient conditions along the Bering Sea shelf (pH 8.0), and those acclimated to a moderately (pH 7.8) and severely (pH 7.5) acidified environments that are projected to occur in surface and bottom waters by the end of this century [15]. Using RNA-Seq, a high-throughput sequencing approach that measures gene-activity, our study provides a snap-shot of system-wide changes in energy allocation due to acidification exposure by identifying genes, their functions, and biological processes that differ in OA-reared crab [56]. Libraries were constructed from at least 13 individuals per treatment, rather than pools of individuals which can obscure genotypedependent variation. Importantly, since the crab used in this experiment were quite tolerant of OA conditions, the molecular mechanisms and pathways described here may be potentially critical to survival in an acidified environment. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_967461 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and gene function and transposon activity of juvenile subarctic crustacean Spencer, Laura H Long, William Christopher Spies, Ingrid B Nichols, Krista M Foy, Robert J Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Arthropoda; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Identification; Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Paralithodes camtschaticus; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, standard deviation; pH, total scale; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type of study In this study, we used functional genomics to examine the molecular response of OA exposed red king crab. We leveraged juveniles that were exposed to (and tolerated) three carbonate chemistry treatments from hatching to the first crab stage (C1), thus capturing transcriptional differences among crab that are reared in historically ambient conditions along the Bering Sea shelf (pH 8.0), and those acclimated to a moderately (pH 7.8) and severely (pH 7.5) acidified environments that are projected to occur in surface and bottom waters by the end of this century [15]. Using RNA-Seq, a high-throughput sequencing approach that measures gene-activity, our study provides a snap-shot of system-wide changes in energy allocation due to acidification exposure by identifying genes, their functions, and biological processes that differ in OA-reared crab [56]. Libraries were constructed from at least 13 individuals per treatment, rather than pools of individuals which can obscure genotypedependent variation. Importantly, since the crab used in this experiment were quite tolerant of OA conditions, the molecular mechanisms and pathways described here may be potentially critical to survival in an acidified environment. |
| title | Seawater carbonate chemistry and gene function and transposon activity of juvenile subarctic crustacean |
| topic | Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aragonite saturation state, standard deviation; Arthropoda; Benthic animals; Benthos; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Identification; Laboratory experiment; North Pacific; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Paralithodes camtschaticus; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH, standard deviation; pH, total scale; Salinity; Salinity, standard deviation; Single species; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); Temperate; Temperature, water; Temperature, water, standard deviation; Treatment; Type of study |
| url | https://doi.org/10.1594/PANGAEA.967461 |