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Bioassays quantifying the adverse effects of three strains of the harmful dinoflagellate Alexandrium pseudogonyaulax on the membrane integrity of the RTgill-W1 cell line of the rainbow trout (Oncorhynchus mykiss)

Fuente: PANGAEA

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Dettagli Bibliografici
Autori principali:	Möller, Kristof, Tillmann, Urban, Pöchhacker, Magdalena, Varga, Elisabeth, Krock, Bernd, Koch, Florian, Harris, Thomas M, Meunier, Cédric Léo
Natura:	Dataset Open Access
Lingua:	en
Pubblicazione:	PANGAEA 2024
Soggetti:	A. pseudogonyaulax isolated from Limfjord; Alexandrium, cell density, equivalent; Alexandrium cells centrifuged off, supernatant diluted with K-medium to obtain different concentrations; Alexandrium pseudogonyaulax, strain; Alexandrium sp.; Bioactive extracellular substances (BECs); Bioassay; Cell titre blue (CTB); CyQUANTTM™ lactate dehydrogenase (LDH) cytotoxicity assay kit, Thermo Fisher Scientific, C20301; measured with cell imaging multi-mode reader, BioTek, Cytation™ 3; Date/time end, experiment; Date/time start, experiment; Extracellular lactate dehydrogenase activity, gill cells of Oncorhynchus mykiss, relative; Incubation duration; Incubation volume; Laboratory experiment; lactate dehydrogenase (LDH); Microwell plate incubation; MULT; Multiple investigations; Oncorhynchus mykiss; phycotoxins; Replicate, biological; Replicate, technical; Species, unique identification; Species, unique identification (Semantic URI); Species, unique identification (URI); T-AP-N; Thyborøn, Limfjord, Denmark; Treatment: toxin; Type of study; viability
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