Temperature responses of different functional traits in Arctic phytoplankton
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| Formato: | Dataset Open Access |
| Lenguaje: | en |
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PANGAEA
2024
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| _version_ | 1867171043095871488 |
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| author | Rehder, Linda Rokitta, Sebastian D Buschmann, Isabelle Jasper, Levke Hoppe, Clara Jule Marie Rost, Björn |
| author_facet | Rehder, Linda Rokitta, Sebastian D Buschmann, Isabelle Jasper, Levke Hoppe, Clara Jule Marie Rost, Björn |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | This study was conducted in autumn/winter 2021/2022 at the laboratory of the Alfred-Wegener-Institute Bremerhaven. To assess temperature response patterns of various functional traits in Arctic phytoplankton species, we cultivated Thalassiosira hyalina, Phaeocystis pouchetii, Micromonas pusilla and Nitzschia frigida at a temperature gradient of 0°C to 14°C under saturating light of 100 µmol photons m-2 s-1 (T. hyalina, P. pouchetii, M. pusilla) or 30 µmol photons m-2 s-1 (N. frigida) and replete nutrient conditions (micronutrients following F/2; 100 µmol L-1 nitrate, 100 µmol L-1 silicate, 6 µmol L-1 phosphate) in dilute batch cultures. Culture bottles were aerated with air containing a pCO2 of 400 µatm ensure a stabilized carbonate chemistry and to avoid sedimentation. After an acclimation period of >7 generation or >10 days, respectively, various functional traits were obtained: specific growth rate, quotas of particulate organic carbon (POC) and nitrogen (PON) as well as chlorophyll a (Chl a), photo-physiology using fast repetition rate fluorometry. Furthermore, the following parameters were calculated from the obtained data: POC:PON, Chl a:POC, POC production rate, photosystem II quantum yield, absolute electron transport rates at in-situ irradiance and maximal irradiance, light use efficiency, light acclimation indices, lifetime of PSII reopening and absorption cross section. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_968670 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Temperature responses of different functional traits in Arctic phytoplankton Rehder, Linda Rokitta, Sebastian D Buschmann, Isabelle Jasper, Levke Hoppe, Clara Jule Marie Rost, Björn Absorption cross section of PSII photochemistry; Calculated; Carbon, organic, particulate, net production, per cell; Carbon, organic, particulate, per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Daily cell counting, Beckmann coulter Counter; Diatom; Electron transport rate, absolute; Experiment; Fast repetition rate fluorometer; Flash combustions technique, CN Analyzer, EuroVector EA 3000; Fluorometry; FRRF; Generation; growth; Growth rate; ice algae; Laboratory; Light saturation; Light use efficiency; Location; Maximal electron transport rate; Medium; Nitrogen, organic, particulate, per cell; Phaeocystis pouchetii; physiology; Phytoplankton; Quantum yield efficiency of photosystem II; Sample ID; Sampling date; Species, unique identification; Species, unique identification (URI); Strain; Temperate; Time constant for the initial part of an single turnover relaxation phase; Time in years; Treatment: light:dark cycle; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: salinity; Treatment: temperature; Type of study This study was conducted in autumn/winter 2021/2022 at the laboratory of the Alfred-Wegener-Institute Bremerhaven. To assess temperature response patterns of various functional traits in Arctic phytoplankton species, we cultivated Thalassiosira hyalina, Phaeocystis pouchetii, Micromonas pusilla and Nitzschia frigida at a temperature gradient of 0°C to 14°C under saturating light of 100 µmol photons m-2 s-1 (T. hyalina, P. pouchetii, M. pusilla) or 30 µmol photons m-2 s-1 (N. frigida) and replete nutrient conditions (micronutrients following F/2; 100 µmol L-1 nitrate, 100 µmol L-1 silicate, 6 µmol L-1 phosphate) in dilute batch cultures. Culture bottles were aerated with air containing a pCO2 of 400 µatm ensure a stabilized carbonate chemistry and to avoid sedimentation. After an acclimation period of >7 generation or >10 days, respectively, various functional traits were obtained: specific growth rate, quotas of particulate organic carbon (POC) and nitrogen (PON) as well as chlorophyll a (Chl a), photo-physiology using fast repetition rate fluorometry. Furthermore, the following parameters were calculated from the obtained data: POC:PON, Chl a:POC, POC production rate, photosystem II quantum yield, absolute electron transport rates at in-situ irradiance and maximal irradiance, light use efficiency, light acclimation indices, lifetime of PSII reopening and absorption cross section. |
| title | Temperature responses of different functional traits in Arctic phytoplankton |
| topic | Absorption cross section of PSII photochemistry; Calculated; Carbon, organic, particulate, net production, per cell; Carbon, organic, particulate, per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Daily cell counting, Beckmann coulter Counter; Diatom; Electron transport rate, absolute; Experiment; Fast repetition rate fluorometer; Flash combustions technique, CN Analyzer, EuroVector EA 3000; Fluorometry; FRRF; Generation; growth; Growth rate; ice algae; Laboratory; Light saturation; Light use efficiency; Location; Maximal electron transport rate; Medium; Nitrogen, organic, particulate, per cell; Phaeocystis pouchetii; physiology; Phytoplankton; Quantum yield efficiency of photosystem II; Sample ID; Sampling date; Species, unique identification; Species, unique identification (URI); Strain; Temperate; Time constant for the initial part of an single turnover relaxation phase; Time in years; Treatment: light:dark cycle; Treatment: light intensity; Treatment: partial pressure of carbon dioxide; Treatment: salinity; Treatment: temperature; Type of study |
| url | https://doi.org/10.1594/PANGAEA.968670 |