Bacterial composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254
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PANGAEA
2024
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| author | Stratmann, Tanja Miranda Montenegro, Mario Luis de Kluijver, Anna Busch, Kathrin Kelly, Michelle Mills, Sadie Schupp, Peter |
| author_facet | Stratmann, Tanja Miranda Montenegro, Mario Luis de Kluijver, Anna Busch, Kathrin Kelly, Michelle Mills, Sadie Schupp, Peter |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | During R/V Sonne cruise SO254, we conducted ex-situ experiments with shallow- and deep-water demo- and hexactinellid sponges from New Zealand. Over a period of 5 hours, the sponges were incubated at in-situ temperature in the light (shallow-water incubation) or in the dark (deep-water incubation), and water samples for dissolved organic carbon (DOC), total dissolved nitrogen (TDN), and fluorescing dissolved organic matter (FDOM) were taken every hour. At the end of the incubations, samples for (biogeochemical) tissue analyses (including fatty acid analyses) were taken of the sponges together with samples for microbial analyses of the bacterial community living inside the sponges. Bacteria community composition of 4 species (number of replicates per species: 1) were analyzed based on DNA extracted from sub-samples taken from the sponges at the end of the incubations. After sequencing, representative amplicon sequence variants (ASVs) were classified taxonomically with the SILVA database. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_971514 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Bacterial composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254 Stratmann, Tanja Miranda Montenegro, Mario Luis de Kluijver, Anna Busch, Kathrin Kelly, Michelle Mills, Sadie Schupp, Peter Acidobacteria; Actinobacteria; Area/locality; Bacteria, phyla; Bacteroidetes; Campaign of event; Chlamydiae; Chloroflexota; Cyanobacteria; Dadabacteria; Date/Time of event; Deinococcus-Thermus; DIVER; EEM; Elevation of event; Entotheonellaeota; Euryarchaeota; Event label; Fibrobacteres; Firmicutes; Gemmatimonadetes; Laboratory code/label; Latitude of event; long-chain fatty acid; Longitude of event; Margulisbacteria; Marinimicrobia; Nitrospinae; Nitrospirae; Pacific; PARAFAC; Patescibacteria; Planctomycetes; PLFA; Pori Bac NewZ; Poribacteria; Proteobacteria; PUFA; Remote operated vehicle; ROV; Sample code/label; Sampling by diver; SO254; SO254_33-1; SO254_36-1; SO254_diver; Sonne_2; South Pacific Ocean; Spirochaetes; sponge; Taxon/taxa; Taxon/taxa, unique identification; Taxon/taxa, unique identification (Semantic URI); Taxon/taxa, unique identification (URI); Tenericutes; Verrucomicrobia During R/V Sonne cruise SO254, we conducted ex-situ experiments with shallow- and deep-water demo- and hexactinellid sponges from New Zealand. Over a period of 5 hours, the sponges were incubated at in-situ temperature in the light (shallow-water incubation) or in the dark (deep-water incubation), and water samples for dissolved organic carbon (DOC), total dissolved nitrogen (TDN), and fluorescing dissolved organic matter (FDOM) were taken every hour. At the end of the incubations, samples for (biogeochemical) tissue analyses (including fatty acid analyses) were taken of the sponges together with samples for microbial analyses of the bacterial community living inside the sponges. Bacteria community composition of 4 species (number of replicates per species: 1) were analyzed based on DNA extracted from sub-samples taken from the sponges at the end of the incubations. After sequencing, representative amplicon sequence variants (ASVs) were classified taxonomically with the SILVA database. |
| title | Bacterial composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254 |
| topic | Acidobacteria; Actinobacteria; Area/locality; Bacteria, phyla; Bacteroidetes; Campaign of event; Chlamydiae; Chloroflexota; Cyanobacteria; Dadabacteria; Date/Time of event; Deinococcus-Thermus; DIVER; EEM; Elevation of event; Entotheonellaeota; Euryarchaeota; Event label; Fibrobacteres; Firmicutes; Gemmatimonadetes; Laboratory code/label; Latitude of event; long-chain fatty acid; Longitude of event; Margulisbacteria; Marinimicrobia; Nitrospinae; Nitrospirae; Pacific; PARAFAC; Patescibacteria; Planctomycetes; PLFA; Pori Bac NewZ; Poribacteria; Proteobacteria; PUFA; Remote operated vehicle; ROV; Sample code/label; Sampling by diver; SO254; SO254_33-1; SO254_36-1; SO254_diver; Sonne_2; South Pacific Ocean; Spirochaetes; sponge; Taxon/taxa; Taxon/taxa, unique identification; Taxon/taxa, unique identification (Semantic URI); Taxon/taxa, unique identification (URI); Tenericutes; Verrucomicrobia |
| url | https://doi.org/10.1594/PANGAEA.971514 |