Biogeochemical and phospholipid-derived fatty acid (PLFA) composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254
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PANGAEA
2024
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| author | Stratmann, Tanja Miranda Montenegro, Mario Luis de Kluijver, Anna Busch, Kathrin Kelly, Michelle Mills, Sadie Schupp, Peter |
| author_facet | Stratmann, Tanja Miranda Montenegro, Mario Luis de Kluijver, Anna Busch, Kathrin Kelly, Michelle Mills, Sadie Schupp, Peter |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | During R/V Sonne cruise SO254, we conducted ex-situ experiments with shallow- and deep-water demo- and hexactinellid sponges from New Zealand. Over a period of 5 hours, the sponges were incubated at in-situ temperature in the light (shallow-water incubation) or in the dark (deep-water incubation), and water samples for dissolved organic carbon (DOC), total dissolved nitrogen (TDN), and fluorescing dissolved organic matter (FDOM) were taken every hour. At the end of the incubations, samples for (biogeochemical) tissue analyses (including fatty acid analyses) were taken of the sponges together with samples for microbial analyses of the bacterial community living inside the sponges. The biogeochemical composition of 4 shallow-water and deep-water sponge species were determined after the cruise by measuring the organic and total carbon and nitrogen content of freeze-dried sponge tissue and the stable isotopic composition (δ13C, δ15N) of the tissue. Phospholipid-derived fatty acids (PLFAs) of freeze-dried sponge tissue were extracted using a modified Bligh-Dyer extraction protocol and subsequently the PLFA concentrations were measured using gas chromatography flame ionization detection (GC-FID). |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_971544 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Biogeochemical and phospholipid-derived fatty acid (PLFA) composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254 Stratmann, Tanja Miranda Montenegro, Mario Luis de Kluijver, Anna Busch, Kathrin Kelly, Michelle Mills, Sadie Schupp, Peter Area/locality; Ash free dry mass; Campaign of event; Carbon, organic; Carbon, total; Date/Time of event; DIVER; Dry mass; EEM; Elevation of event; Event label; Laboratory code/label; Latitude of event; long-chain fatty acid; Longitude of event; Nitrogen, total; Pacific; PARAFAC; Phospholipid fatty acids; PLFA; Pori Bac NewZ; PUFA; Remote operated vehicle; ROV; Sample code/label; Sampling by diver; SO254; SO254_33-1; SO254_36-1; SO254_diver; Sonne_2; South Pacific Ocean; sponge; Taxon/taxa; Taxon/taxa, unique identification; Taxon/taxa, unique identification (Semantic URI); Taxon/taxa, unique identification (URI); Volume; Wet mass; δ13C, organic carbon; δ13C, total carbon; δ15N, total nitrogen During R/V Sonne cruise SO254, we conducted ex-situ experiments with shallow- and deep-water demo- and hexactinellid sponges from New Zealand. Over a period of 5 hours, the sponges were incubated at in-situ temperature in the light (shallow-water incubation) or in the dark (deep-water incubation), and water samples for dissolved organic carbon (DOC), total dissolved nitrogen (TDN), and fluorescing dissolved organic matter (FDOM) were taken every hour. At the end of the incubations, samples for (biogeochemical) tissue analyses (including fatty acid analyses) were taken of the sponges together with samples for microbial analyses of the bacterial community living inside the sponges. The biogeochemical composition of 4 shallow-water and deep-water sponge species were determined after the cruise by measuring the organic and total carbon and nitrogen content of freeze-dried sponge tissue and the stable isotopic composition (δ13C, δ15N) of the tissue. Phospholipid-derived fatty acids (PLFAs) of freeze-dried sponge tissue were extracted using a modified Bligh-Dyer extraction protocol and subsequently the PLFA concentrations were measured using gas chromatography flame ionization detection (GC-FID). |
| title | Biogeochemical and phospholipid-derived fatty acid (PLFA) composition of shallow-water and deep-water demo- and hexactinellid sponges from New Zealand collected during SONNE cruise SO254 |
| topic | Area/locality; Ash free dry mass; Campaign of event; Carbon, organic; Carbon, total; Date/Time of event; DIVER; Dry mass; EEM; Elevation of event; Event label; Laboratory code/label; Latitude of event; long-chain fatty acid; Longitude of event; Nitrogen, total; Pacific; PARAFAC; Phospholipid fatty acids; PLFA; Pori Bac NewZ; PUFA; Remote operated vehicle; ROV; Sample code/label; Sampling by diver; SO254; SO254_33-1; SO254_36-1; SO254_diver; Sonne_2; South Pacific Ocean; sponge; Taxon/taxa; Taxon/taxa, unique identification; Taxon/taxa, unique identification (Semantic URI); Taxon/taxa, unique identification (URI); Volume; Wet mass; δ13C, organic carbon; δ13C, total carbon; δ15N, total nitrogen |
| url | https://doi.org/10.1594/PANGAEA.971544 |