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| Format: | Dataset Open Access |
| Language: | en |
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PANGAEA
2024
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| Online Access: | https://doi.org/10.1594/PANGAEA.971688 |
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| _version_ | 1867168230034898944 |
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| author | Devresse, Quentin Becker, Kevin W Engel, Anja |
| author_facet | Devresse, Quentin Becker, Kevin W Engel, Anja |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We collected in situ marine snow aggregates with a Marine Snow Catcher (MSC). The MSC allows studying the composition of suspended and fast-sinking organic matter from intact in situ formed aggregates (Riley et al. 2012). After deployment, the MSC was left vertically on deck for three hours, seawater containing suspended organic matter was collected from the top part of the MSC and fast-sinking organic matter was picked manually from the MSC base. The MSC was deployed within the mixed layer depth in the oxygen minimum zone (~400 m depth). Duplicate samples for particulate organic carbon (POC) and organic nitrogen (PON) were filtered onto pre-combusted (450 ◦C for 5 h) Whatman GF/F filters (25 mm, 0.7 µm) under low pressure (< 200 mbar). Filters were frozen at −20 °C and stored until analysis. Prior to analysis, filters were acid-fumed (37% HCl for 24 h) in order to remove inorganic carbon and dried at 40°C for 24 h. Subsequently, filters were wrapped in tin cups (8×8×15 mm), combusted and analyzed according to Sharp (1974) using an elemental analyzer (Euro EA). We determined high-molecular-weight (>1 kDa) particulate combined carbohydrates (pCCHO) based on Engel and Händel (2011) and particulate hydrolysable amino acids (pHAA) based on Lindroth and Mopper (1979) and Dittmar et al., (2009). The analysis of pCCHO detected 11 monomers: and the pHAA analysis classified 13 monomers. The calculations for the carbon and nitrogen contents of pCCHO and pHAA were based on carbon and nitrogen atoms contained in the identified monomers. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_971688 |
| institution | PANGAEA |
| language | en |
| publishDate | 2024 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Particulate amino acid and carbohydrates and particulate organic carbon and nitrogen in suspended and sinking organic matter from METEOR cruise M156 Devresse, Quentin Becker, Kevin W Engel, Anja Alanine; amino acids; Amino acids; Arabinose; Arginine; Aspartic acid and Asparagine; Canarias Sea; Carbohydrate; Carbohydrates, particulate; Carbon, organic, particulate; DATE/TIME; Deployment number; DEPTH, water; Event label; Fucose; Galactosamine; Galactose; Galacturonic acid; gamma-Aminobutyric acid; Gluconic acid; Glucosamine; Glucose; Glucuronic acid; Glutamine and Glutamic acid; Glycine; High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000; High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260; Isoleucine; LATITUDE; Leucine; LONGITUDE; M156; M156_108-1; M156_12-1; M156_126-1; M156_139-1; M156_152-1; M156_156-1; M156_162-1; M156_18-1; M156_2-1; M156_23-1; M156_36-1; M156_45-1; M156_51-1; M156_57-1; M156_65-1; M156_74-1; M156_80-1; M156_88-1; M156_95-1; M156_99-1; Mannose/Xylose; marine snow; Marine snow catcher; Meteor (1986); MSC; Muramic acid; Nitrogen, organic, particulate; North Atlantic Ocean; Phenylalanine; POC; PON; REEBUS; Rhamnose; Role of Eddies for the Carbon Pump in Coastal upwelling Areas; Serine; Station label; Threonine; Tyrosine; Valine We collected in situ marine snow aggregates with a Marine Snow Catcher (MSC). The MSC allows studying the composition of suspended and fast-sinking organic matter from intact in situ formed aggregates (Riley et al. 2012). After deployment, the MSC was left vertically on deck for three hours, seawater containing suspended organic matter was collected from the top part of the MSC and fast-sinking organic matter was picked manually from the MSC base. The MSC was deployed within the mixed layer depth in the oxygen minimum zone (~400 m depth). Duplicate samples for particulate organic carbon (POC) and organic nitrogen (PON) were filtered onto pre-combusted (450 ◦C for 5 h) Whatman GF/F filters (25 mm, 0.7 µm) under low pressure (< 200 mbar). Filters were frozen at −20 °C and stored until analysis. Prior to analysis, filters were acid-fumed (37% HCl for 24 h) in order to remove inorganic carbon and dried at 40°C for 24 h. Subsequently, filters were wrapped in tin cups (8×8×15 mm), combusted and analyzed according to Sharp (1974) using an elemental analyzer (Euro EA). We determined high-molecular-weight (>1 kDa) particulate combined carbohydrates (pCCHO) based on Engel and Händel (2011) and particulate hydrolysable amino acids (pHAA) based on Lindroth and Mopper (1979) and Dittmar et al., (2009). The analysis of pCCHO detected 11 monomers: and the pHAA analysis classified 13 monomers. The calculations for the carbon and nitrogen contents of pCCHO and pHAA were based on carbon and nitrogen atoms contained in the identified monomers. |
| title | Particulate amino acid and carbohydrates and particulate organic carbon and nitrogen in suspended and sinking organic matter from METEOR cruise M156 |
| topic | Alanine; amino acids; Amino acids; Arabinose; Arginine; Aspartic acid and Asparagine; Canarias Sea; Carbohydrate; Carbohydrates, particulate; Carbon, organic, particulate; DATE/TIME; Deployment number; DEPTH, water; Event label; Fucose; Galactosamine; Galactose; Galacturonic acid; gamma-Aminobutyric acid; Gluconic acid; Glucosamine; Glucose; Glucuronic acid; Glutamine and Glutamic acid; Glycine; High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000; High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260; Isoleucine; LATITUDE; Leucine; LONGITUDE; M156; M156_108-1; M156_12-1; M156_126-1; M156_139-1; M156_152-1; M156_156-1; M156_162-1; M156_18-1; M156_2-1; M156_23-1; M156_36-1; M156_45-1; M156_51-1; M156_57-1; M156_65-1; M156_74-1; M156_80-1; M156_88-1; M156_95-1; M156_99-1; Mannose/Xylose; marine snow; Marine snow catcher; Meteor (1986); MSC; Muramic acid; Nitrogen, organic, particulate; North Atlantic Ocean; Phenylalanine; POC; PON; REEBUS; Rhamnose; Role of Eddies for the Carbon Pump in Coastal upwelling Areas; Serine; Station label; Threonine; Tyrosine; Valine |
| url | https://doi.org/10.1594/PANGAEA.971688 |