Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2025
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| _version_ | 1867168577756332032 |
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| author | Cardoso, Isabel Bartsch, Inka Valentin, Klaus Steinhagen, Sophie Buck, Bela Hieronymus Hofmann, Laurie C |
| author_facet | Cardoso, Isabel Bartsch, Inka Valentin, Klaus Steinhagen, Sophie Buck, Bela Hieronymus Hofmann, Laurie C |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Degradation in Ulva lacinulata and reproduction in Ulva compressa were observed and described for four weeks. The natural occurrence of protoplasts in both species was found. Protoplast yields were calculated each week for several weeks. At the beginning of the experiment, heterogeneity of the thallus was visible under the microscope. The diameter of the cells in the thalli that later became protoplasts were measured. A germination experiment was performed after protoplasts were collected. Germination rates were calculated based on the number of individuals visible by the naked eye at the end of the experiment. 2 % (U. lacinulata) and 4 % (U. compressa) of protoplasts germinated into two morphologies: unattached germlings and unattached discs. Because discs released gametes early in their development, their diameter was measured to estimate the gametes yields produced at the end of the experiment. During degradation of U. lacinulata, we found an increase in biomass after the first two weeks of the experiment. Fresh weight measurements were taken each week and relative growth rates were calculated. Our results provide the first evidence of the natural production of protoplasts in Ulva spp. and estimate that higher seeding yields can be obtained by natural protoplast production than direct gametogenesis. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_972125 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions Cardoso, Isabel Bartsch, Inka Valentin, Klaus Steinhagen, Sophie Buck, Bela Hieronymus Hofmann, Laurie C degradation; Laboratory experiment; pincuschion; protoplasts; Reproduction; Ulva; Ulva compressa; Ulva lacinulata Degradation in Ulva lacinulata and reproduction in Ulva compressa were observed and described for four weeks. The natural occurrence of protoplasts in both species was found. Protoplast yields were calculated each week for several weeks. At the beginning of the experiment, heterogeneity of the thallus was visible under the microscope. The diameter of the cells in the thalli that later became protoplasts were measured. A germination experiment was performed after protoplasts were collected. Germination rates were calculated based on the number of individuals visible by the naked eye at the end of the experiment. 2 % (U. lacinulata) and 4 % (U. compressa) of protoplasts germinated into two morphologies: unattached germlings and unattached discs. Because discs released gametes early in their development, their diameter was measured to estimate the gametes yields produced at the end of the experiment. During degradation of U. lacinulata, we found an increase in biomass after the first two weeks of the experiment. Fresh weight measurements were taken each week and relative growth rates were calculated. Our results provide the first evidence of the natural production of protoplasts in Ulva spp. and estimate that higher seeding yields can be obtained by natural protoplast production than direct gametogenesis. |
| title | Naturally occurring protoplasts in two Ulva spp. - Disc sizes from discs grown from protoplasts, after 35 days of germination, under culture conditions |
| topic | degradation; Laboratory experiment; pincuschion; protoplasts; Reproduction; Ulva; Ulva compressa; Ulva lacinulata |
| url | https://doi.org/10.1594/PANGAEA.972125 |