Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina under different nitric oxide (NO) supply rates
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
2025
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| _version_ | 1867169163105009664 |
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| author | Takeda, Shigenobu |
| author_facet | Takeda, Shigenobu |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina isolated from Japanese coastal waters were monitored under different nitric oxide (NO) supply rates using a variable-volume repeated fed-batch (VRFB) culture system. Culture experiments for S. marinoi-dohrnii complex were performed at three NO levels: low, medium, and high. The average NO supply rates at the low, medium, and high NO levels were 1.4, 5.4, and 28 pM/s, respectively. In the C. marina culture experiments, 2.5 pM/s NO supply rate (low-medium treatment) was used instead of 28 pM/s. In both experiments, a treatment feeding deoxygenated f/2 medium without NO was used as the control. The S. marinoi-dohrnii complex and C. marina were cultured in the VRFB culture system for 3 days. To examine the recovery from NO-induced growth inhibition, phytoplankton growth was monitored for several days after the termination of 3-day NO supply. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_972843 |
| institution | PANGAEA |
| language | en |
| publishDate | 2025 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina under different nitric oxide (NO) supply rates Takeda, Shigenobu Bisan-Seto, Seto Inland Sea, Naoshima, Kagawa, Japan; Calculated; Cell; Chattonella; Date/time end, experiment; Date/time start, experiment; Event label; Experimental treatment; Fluorescence, in vivo; Growth rate; Inverted microscope, Olympus, CKX-41; Laboratory experiment; Laboratory fluorometer, Turner, Trilogy; with a chlorophyll in-vivo module; nitric oxide; NO sensor, World Precision Instruments, ISO-NOPF200; Free-radical analyzer, World Precision Instruments, TBR4100; Off Kishiwada, Osaka Bay, Osaka, Japan; Origin of Chattonella marina isolates; Origin of S. marinoi-dohrnii complex isolates; Peristaltic pump, Masterflex, L/S 7550-50; Phytoplankton; Skeletonema; Species; Strain; Time, incubation; Treatment: feed solution, supply rate; Treatment: nitric oxide, supply rate; Type of study; Variable-volume repeated fed-batch culture system; VRFB; Water sampler Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina isolated from Japanese coastal waters were monitored under different nitric oxide (NO) supply rates using a variable-volume repeated fed-batch (VRFB) culture system. Culture experiments for S. marinoi-dohrnii complex were performed at three NO levels: low, medium, and high. The average NO supply rates at the low, medium, and high NO levels were 1.4, 5.4, and 28 pM/s, respectively. In the C. marina culture experiments, 2.5 pM/s NO supply rate (low-medium treatment) was used instead of 28 pM/s. In both experiments, a treatment feeding deoxygenated f/2 medium without NO was used as the control. The S. marinoi-dohrnii complex and C. marina were cultured in the VRFB culture system for 3 days. To examine the recovery from NO-induced growth inhibition, phytoplankton growth was monitored for several days after the termination of 3-day NO supply. |
| title | Growth responses of Skeletonema marinoi-dohrnii complex and Chattonella marina under different nitric oxide (NO) supply rates |
| topic | Bisan-Seto, Seto Inland Sea, Naoshima, Kagawa, Japan; Calculated; Cell; Chattonella; Date/time end, experiment; Date/time start, experiment; Event label; Experimental treatment; Fluorescence, in vivo; Growth rate; Inverted microscope, Olympus, CKX-41; Laboratory experiment; Laboratory fluorometer, Turner, Trilogy; with a chlorophyll in-vivo module; nitric oxide; NO sensor, World Precision Instruments, ISO-NOPF200; Free-radical analyzer, World Precision Instruments, TBR4100; Off Kishiwada, Osaka Bay, Osaka, Japan; Origin of Chattonella marina isolates; Origin of S. marinoi-dohrnii complex isolates; Peristaltic pump, Masterflex, L/S 7550-50; Phytoplankton; Skeletonema; Species; Strain; Time, incubation; Treatment: feed solution, supply rate; Treatment: nitric oxide, supply rate; Type of study; Variable-volume repeated fed-batch culture system; VRFB; Water sampler |
| url | https://doi.org/10.1594/PANGAEA.972843 |